| 姜黄素对体外培养的增生性瘢痕成纤维细胞的生物学作用 |
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| 引用本文： | 胡晓龙,胡大海,计鹏,蔡维霞,白晓智,杨崇志,韩勇彬.姜黄素对体外培养的增生性瘢痕成纤维细胞的生物学作用[J].第四军医大学学报,2009,30(1):76-79. |
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| 作者姓名： | 胡晓龙 胡大海 计鹏 蔡维霞 白晓智 杨崇志 韩勇彬 |
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| 作者单位： | 第四军医大学西京医院烧伤与皮肤外科,陕西,西安,710033
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| 摘 要： | 目的：观察姜黄素体外抗增生性瘢痕的作用．方法：以增生性瘢痕成纤维细胞为靶细胞，分别用不同浓度的姜黄素培养液培养，MTT法测定细胞的增殖情况，透射电镜进行形态学检测，流式细胞仪技术结合PI及Annexin V双标记染色检测姜黄素的促凋亡作用，实时荧光定量聚合酶链反应检测Ⅰ，Ⅲ型前胶原及Bcl-2 mRNA的表达情况．结果：姜黄素对增生性瘢痕成纤维细胞的增殖有抑制作用；透射电镜观察发现，处理组凋亡细胞增多，凋亡细胞表现出典型的超微结构特征；流式细胞仪检测发现，随着药物浓度的增加，增生性瘢痕成纤维细胞的凋亡率也在逐渐升高；实时荧光定量聚合酶链反应检测结果显示，姜黄素处理后Ⅰ、Ⅲ型前胶原及Bcl-2 mRNA的表达水平均下调．结论：姜黄素具有一定的体外抗增生性瘢痕的作用．
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| 关 键 词： | 姜黄素 增生性瘢痕 成纤维细胞 凋亡 Ⅰ型前胶原 Ⅲ型前胶原 Bcl-2 |
Biological effects of curcumin on fibroblasts derived from hypertrophic scars in vitro |
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| HU Xiao-Long,HU Da-Hai,JI Peng,CAI Wei-Xia,BAI Xiao-Zhi,YANG Chong-Zhi,HAN Yong-Bin.Biological effects of curcumin on fibroblasts derived from hypertrophic scars in vitro[J].Journal of the Fourth Military Medical University,2009,30(1):76-79. |
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| Authors: | HU Xiao-Long HU Da-Hai JI Peng CAI Wei-Xia BAI Xiao-Zhi YANG Chong-Zhi HAN Yong-Bin |
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| Institution: | HU Xiao-Long,HU Da-Hai,JI Peng,CAI Wei-Xia,BAI Xiao-Zhi,YANG Chong-Zhi,HAN Yong-BinDepartment of Burns and Dermatological Surgery,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM:To evaluate the inhibitory effects of the curcumin against hypertrophic scar in vitro. METHODS: Hypertrophic scar fibroblasts (HSFB), as target cells, were exposed to different concentrations of curcumin respectively. The proliferation of human HSFB was evaluated by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The morphological characters of hypertrophic scar fibroblasts were analyzed by electron microscopy. The apoptosis induced by curcumin were studied by combination of flow cytometry (FCM) ... |
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| Keywords: | Bcl-2 |
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