| U266细胞在组蛋白去乙酰化酶抑制剂MS-275诱导下凋亡观察 |
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| 引用本文： | 马健,赵名,于晓妉,王志红.U266细胞在组蛋白去乙酰化酶抑制剂MS-275诱导下凋亡观察[J].第四军医大学学报,2009,30(9):835-838. |
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| 作者姓名： | 马健 赵名 于晓妉 王志红 |
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| 作者单位： | 马健,王志红,MA Jian,WANG Zhi-Hong(解放军总医院第一附属医院血液科,北京,100037);赵名,于晓妉,ZHAO Ming,YU Xiao-Dan(军事医学科学院基础医学研究所,北京,100850)
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| 摘 要： | 目的：研究组蛋白去乙酰化酶抑制剂MS-275对人多发性骨髓瘤U266细胞的增殖和凋亡的影响.方法：台盼蓝拒染法观察MS-275对细胞活力的影响;瑞氏—姬姆萨染色观察药物对细胞形态学变化;流式细胞仪分析细胞周期;West-ernBlot检测凋亡信号通路中Caspase-3,Caspase-8,Caspase-9活化及蛋白聚ADP核糖聚合酶poly（ADP-ribose）polymer-ase,PARP]裂解情况.结果：MS-275呈时间和剂量依赖性抑制U266细胞增殖,阻断细胞周期于G0/G1期.MS-275作用48h的IC50为1.39μmol/L;2μmol/LMS-275作用细胞24h后,G0/G1期64.57%;36h占82.20%.瑞氏—姬姆萨染色显示细胞发生明显变化.WesternBlot检测表明MS-275作用U266细胞后,Caspase-3,Caspase-8,Caspase-9被裂解活化,其底物蛋白聚ADP核糖聚合酶发生剪切,细胞发生凋亡.结论：MS-275可抑制细胞增殖,并使细胞阻滞在G0/G1期,通过激活细胞内外2条凋亡级联信号通路诱导U266细胞凋亡.
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| 关 键 词： | 组蛋白去乙酰化酶抑制剂 多发性骨髓瘤 细胞系U266 MS-275 凋亡 |
Effects of histone deacetylase inhibitor MS-275 in vitro on apoptosis of U266 cell line |
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| MA Jian,ZHAO Ming,YU Xiao-Dan,WANG Zhi-Hong.Effects of histone deacetylase inhibitor MS-275 in vitro on apoptosis of U266 cell line[J].Journal of the Fourth Military Medical University,2009,30(9):835-838. |
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| Authors: | MA Jian ZHAO Ming YU Xiao-Dan WANG Zhi-Hong |
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| Institution: | MA Jian1,ZHAO Ming2,YU Xiao-Dan2,WANG Zhi-Hong11Department of Hematology,First Affiliated Hospital,PLA General Hospital,Beijing 100037,China,2Department of Pathobiology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | AIM:To study the effect of MS-275, an inhibitor of histone deacetylase, on the growth and apoptosis of myeloma cell line U266. METHODS: U266 cell was cultured in RPMI-1640 in the presence of MS-275 and the cell viability was evaluated by Trypan blue exclusion assay and cell count. The cell morphological change was observed with Wright-Giemsa staining. The cell cycle was analyzed by flow cytometry and the proteins of poly(ADP-ribose) polymerase(PARP), Caspase-3, Caspase-8 and Caspase-9 were detected by Weste... |
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| Keywords: | MS-275 |
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