| 顺铂对人宫颈癌Hela细胞超弱发光影响的观察 |
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| 引用本文： | 杨海宁,惠延平,辛晓燕,黄艳红,王波,杨慧宁.顺铂对人宫颈癌Hela细胞超弱发光影响的观察[J].第四军医大学学报,2009,30(5):393-396. |
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| 作者姓名： | 杨海宁 惠延平 辛晓燕 黄艳红 王波 杨慧宁 |
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| 作者单位： | 杨海宁,YANG Hai-Ning(第四军医大学西京医院病理科;第四军医大学西京医院妇产科);惠延平,HUI Yan-Ping(第四军医大学西京医院病理科);辛晓燕,黄艳红,XIN Xiao-Yan,HUANG Yan-Hong(第四军医大学西京医院妇产科);王波,WANG Bo(第四军医大学军事预防医学系流行病学教研室,陕西,西安,710033);杨慧宁,YANG Hui-Ning(第四军医大学武警总医院感染控制科,北京,100039)
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| 摘 要： | 目的：探讨人宫颈癌Hela细胞株经顺铂（DDP）作用后其超弱发光与细胞增殖活性变化的关系．方法：选择DDP诱导人宫颈癌Hela细胞株，比较人宫颈癌Hela，Hela＋DDP细胞株形态变化．MTT比色法、流式细胞仪检测细胞生长周期变化及凋亡情况；用IFFM-D型流动式化学发光仪检测Hela，Hela＋DDP细胞的超弱发光强度变化．结果：DDP对Hela细胞有生长抑制作用，并呈时间和浓度依赖性，其48h的Ic5。值为3mg,／L，当DDP浓度在3mg／L以下时，对Hela细胞无明显毒性作用，超过此浓度时，其毒性呈剂量效应关系（P〈0．01）．流式细胞仪结果表明，与Hela细胞相比较，Hela＋DDP细胞G2期细胞数增多，而G1，S期的细胞数明显减少（P〈0．01）；细胞凋亡率在24，48，72h分别为（11．4±5．8）％，（21．8±7．9）％，32．5±11．6）％．在1×10^-4mol／L鲁米诺及3mL／L双氧水（H2O2）条件下超弱发光强度随着时间的变化，Hela＋DDP细胞明显降低（P〈0．01）．结论：在DDP非毒性剂量作用后，Hela＋DDP细胞超弱发光强度降低，提示超弱发光检测可能作为筛选敏感化疗药物的一项指标．
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| 关 键 词： | 宫颈肿瘤 超微弱发光 细胞凋亡 顺铂 |
Effect of DDP treatment on ultraweak luminescence in human cervical carcinoma HeLa line |
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| YANG Hai-Ning,HUI Yan-Ping,XIN Xiao-Yan,HUANG Yan-Hong,WANG Bo,YANG Hui-Ning.Effect of DDP treatment on ultraweak luminescence in human cervical carcinoma HeLa line[J].Journal of the Fourth Military Medical University,2009,30(5):393-396. |
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| Authors: | YANG Hai-Ning HUI Yan-Ping XIN Xiao-Yan HUANG Yan-Hong WANG Bo YANG Hui-Ning |
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| Institution: | YANG Hai-Ning1,2,HUI Yan-Ping1,XIN Xiao-Yan2,HUANG Yan-Hong2,WANG Bo3,YANG Hui-Ning4 1Department of Pathology,2Department of Obstetrics and Gynecology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,3Department of Epidemiology,School of Preventive Medicine,4Department of Infection Control,Armed Police General Hospital,Beijing 100039 |
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| Abstract: | AIM: To study the changes of human cervical carcinoma Hela line in ultraweak luminescence and the proliferation activation following DDP treatment.METHODS: Hela cells were selected and exposed to different concentrations of DDP.The activation of the cell line,the percentage of apoptosis and the changes of cell cycle were detected by MTT and flow cytometry at different time points.The intensity of ultraweak luminescence was examined by IFFM-D chemiluminescence analysis.RESULTS: DDP inhibited the growth of He... |
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| Keywords: | cervix neoplasm ultra weak luminescence apoptosis DDP |
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