| 一种新的鼠大脑局部缺血模型 |
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| 引用本文： | 王跃民,谢安,臧益民,朱妙章.一种新的鼠大脑局部缺血模型[J].第四军医大学学报,2001,22(3):284-286. |
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| 作者姓名： | 王跃民 谢安 臧益民 朱妙章 |
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| 作者单位： | 第四军医大学基础部生理学教研室, |
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| 基金项目： | 国家自然科学基金资助项目 !(3 9970 2 0 4) |
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| 摘 要： | 目的 制成鼠大脑局部缺血模型。方法 采用左侧颈动脉离心端插管，灌注一定压力和流量的生理盐水。结果 ①美蓝染色显示大脑左侧大部分区域被染蓝，除皮层着色较浅外，其余部位着色较深。②体感诱发电位在灌注后30min时，其幅度超过灌注前20％。结论 染色说明大脑左侧为缺血区，体感诱发电位幅度的增高，表明大脑处于兴奋性损伤状态。证实了此方法制成的大脑局部缺血可靠，且省时省力简便易行。
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| 关 键 词： | 脑缺血 诱发电位 动物疾病模型 大鼠 |
| 文章编号： | 1000-2790(2001)03-0284-03 |
| 修稿时间： | 1999年11月5日 |
A new focal cerebral ischemia model of rats |
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| WANG Yue Min,XIE An,ZANG Yi Min,ZHU Miao Zhang.A new focal cerebral ischemia model of rats[J].Journal of the Fourth Military Medical University,2001,22(3):284-286. |
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| Authors: | WANG Yue Min XIE An ZANG Yi Min ZHU Miao Zhang |
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| Institution: | WANG Yue Min,XIE An,ZANG Yi Min,ZHU Miao Zhang Department of Physiology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM To make a new focal cerebral ischemia model of rats. METHODS By perfusing normal saline from the catheter inserted in the left common carotid artery with certain pressure and flow. RESULTS Methylene blue staining showed that most areas in left cerebrum became dark blue except that cortex became slight blue. 30 min after infusion, the amplitude of somato sensory evoked potential (SEP) increases by more than 20%. CONCLUSION Staining indicates that left cerebrum becomes ischemia. The augment of SEP amplitude says that cerebrum is in excitotoxic injury state. It has been proved that this regional cerebral ischemia model is reliable and easy to carry out. |
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| Keywords: | cerebral ischemia evoked potential animal disease models |
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