端侧神经吻合口轴突再生的形态学依据

目的 观察感觉神经端侧神经吻合口的轴突再生及生长情况,探索索侧吻合的可行性。方法 以兔耳大神经干端侧吻合模型,采用抗神经丝单克隆抗体免疫组化,葡萄糖氧化酶－ＤＡＢ－硫酸镍铵加强染色法体端侧神经吻合口进行显微观察。结果 ７只动物的切片中匀可见相当数量的再生轴突通过端侧吻合口,其数量与吻合口质量有密切相关,再生轴突来自被吻合的正常耳大神经,长入溃变移植神经段内,原溃变纤维痕迹消失,结论 表明感觉神经端

The morphologic evidence of axon regeneration at the attached site of end-to-side nerve anastomosis

AIM: The purpose of present study is to evaluatethe axon regeneration at the attached site in order to explorethe possibility of application of sensory nerve end-to-sideanastomosis. METHODS: Antineurofilament immunohistochemical method was used to visualize the regenerated axons at the attached site after the sensory nerve segment wasanastomosised to the normal great auricular nerve by end-toside model in rabbits. RESULTS: The regenerated axonswere found having passed through the attached region in all specimens. The number of regenerated axons in each animal was related to the quality of the anastomosis. The axons camefrom the normal great auricular nerve, grew into the attached degenerated nerve segment, and replaced the degeneratednerve fibers. CONCLUSION: These results suggest that sensory nerve a-cons can regenerate following the end-to-sideanastomosis.