MODS大鼠外周血中PPARγ与TNF-α和IL-10动态变化的研究

目的：探讨MODS大鼠外周血中过氧化物酶体增殖物激活受体γ（PPARγ）与TNF-α,IL-10动态变化的关系,并观察PPARγ能否调控炎症反应,即能否保持IL-10/TNF-α比值的稳定.方法：健康雄性SD大鼠96只,随机分为4组：①正常对照组;②LPS刺激组;③罗格列酮（ROSI）预处理组;④选择性拮抗剂2-氯-5-硝基苯胺（GW9662）预处理组.每组又分为1,3,5,7h组.免疫细胞化学结合图像分析检测外周血单个核细胞内PPARγ的表达,利用酶联免疫吸附剂测定法检测外周血中TNF-α及IL-10的水平.结果：①ROSI预处理组TNF-α各时间点的值较LPS刺激组低（P〈0.05）.GW9662预处理组TNF-α各时间点的值较LPS刺激组高（P〈0.05）.ROSI预处理组比同时相LPS刺激组IL-10的水平高（P〈0.05）.GW9662预处理组与LPS刺激组IL-10的变化趋势基本相同,但5h及7h组IL-10的水平比同时相的LPS刺激组低（P〈0.05）.②相关分析表明,外周血单个核细胞内PPARγ和TNF-α在ROSI预处理组、GW9662预处理组呈显著负相关ROSI预处理组（r=-0.635,P〈0.05）;GW9662预处理组（r=-0.725,P〈0.05）].外周血单个核细胞内PPARγ和IL-10在ROSI预处理组、GW9662预处理组呈显著正相关ROSI预处理组（r=0.605,P〈0.05）;GW9662预处理组（r=0.645,P〈0.05）].③ROSI预处理组IL-10/TNF-α值与同时相正常对照组相比无显著差异.GW9662预处理组IL-10/TNF-α值明显降低,与同时相LPS刺激组相比有显著差异（P〈0.05）.结论：PPARγ可降低MODS大鼠外周血中TNF-α的水平,PPARγ升高MODS大鼠外周血中IL-10的水平,PPARγ对MODS大鼠有保护作用.

Relationship of dynamic changes between PPARγ and TNF-α and IL-10 in peripheral blood of MODS rats

AIM：To investigate the relationship of dynamic changes between peroxisome proliferator-activated receptor γ （PPARγ） and TNF-α and IL-10 in peripheral blood of multiple organ dysfunction syndrome （MODS） rats. METHODS：Ninety-six male SD rats were divided randomly into 4 groups：Normal control group,endotoxin （LPS） group,rosiglitazone （ROSI） pretreatment group and PPARγ antagonist GW9662 pretreatment group. Each group was further divided into 4 groups according to different observation time point respectively of 1,3,5 and 7 h. Immunocytochemical detection and image analysis were used to detect the PPARγ expression in peripheral blood mononuclear cells. Levels of TNF-α and IL-10 were detected by enzyme-linked immunosorbent assay. RESULTS：The levels of PPARγ in each time-related subgroups in ROIS pretreatment group were all higher than those in LPS group （P〈0.05）. The level of PPARγ was positively correlated to TNF-α （r=-0.635,P〈0.05） and negatively correlated to IL-10 （r=0.605,P〈0.05）. The levels of PPARγ in each time-related subgroups of GW9662 pretreatment group were higher （P〈0.05） than those in LPS group. The level of PPARγ was negatively correlated to TNF-α （r=-0.725,P〈0.05）and positively correlated to IL-10 （r=0.645,P〈0.05）. The ratio of IL-10/TNF-α in ROSI pretreatment group showed no significant difference （P〉0.05） compared with that in normal control group. In GW9662 pretreatment group,the ratio of IL-10/TNF-α was significantly reduced compared with that in LPS group （P〈0.05）. CONCLUSION：PPARγ exerts some protective effect on MODS rats by the immune adjustment of anti-inflammatory/pro-inflammatory mechanism.