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A Comparative Study on Rat Intestinal Epithelial Cells and Resident Gut Bacteria (ii) Effect of Arsenite
作者姓名:Upreti RK  Kannan A  Shrivastava R  Chaturvedi UC
作者单位:Biomembrane Toxicology Laboratory, Industrial Toxicology Research Centre, M. G. Marg, P. B. No. 80, Lucknow - 226001, India
摘    要:Objective In order to use facultative gut bacteria as an alternate to animals for the initial gastrointestinal toxicity screening of heavy metals, a comparative study on rat intestinal epithelial cells and resident gut bacteria was undertaken. Methods in vitro growth rate of four gut bacteria, dehydrogenase (DHA) and esterase (EA) activity test, intestinal epithelial and bacterial cell membrane enzymes and in situ effect of arsenite were analysed. Results Growth profile of mixed resident population of gut bacteria and pure isolates of Escherichia coli, Pseudomonas sp., Lactobacillus sp., and Staphylococcus sp. revealed an arsenite (2-20 ppm) concentration-dependent inhibition. The viability pattern of epithelial cells also showed similar changes. DHA and EA tests revealed significant inhibition (40%-72%) with arsenite exposure of 5 and 10 ppm in isolated gut bacteria and epithelial cells. Decrease in membrane alkaline phosphatase and Ca^2+-Mg^2+-ATPase activities was in the range of 33%-55% in four bacteria at the arsenite exposure of 10 ppm, whereas it was 60%-65% in intestinal epithelial villus cells, in situ incubation of arsenite using intestinal loops also showed more or less similar changes in membrane enzymes of resident gut bacterial population and epithelial cells. Conclusion The results indicate that facultative gut bacteria can be used as suitable in vitro model for the preliminary screening of arsenical gastrointestinal cytotoxic effects.

关 键 词:肠上皮细胞  肠内细菌  亚砷酸盐  小鼠  动物实验
收稿时间:2004-12-20

A comparative study on rat intestinal epithelial cells and resident gut bacteria (ii) effect of arsenite
Upreti RK,Kannan A,Shrivastava R,Chaturvedi UC.A comparative study on rat intestinal epithelial cells and resident gut bacteria (ii) effect of arsenite[J].Biomedical and Environmental Sciences,2006,19(2):77-86.
Authors:Upreti Raj K  Kannan A  Shrivastava Richa  Chaturvedi U C
Institution:Biomembrane Toxicology Laboratory, Industrial Toxicology Research Centre, M. G. Marg, P. B. No. 80, Lucknow-226001, India. upretirk@rediffmail.com
Abstract:Objective In order to use facultative gut bacteria as an alternate to animals for the initial gastrointestinal toxicity screening of heavy metals, a comparative study on rat intestinal epithelial cells and resident gut bacteria was undertaken. Methods in vitro growth rate of four gut bacteria, dehydrogenase (DHA) and esterase (EA) activity test, intestinal epithelial and bacterial cell membrane enzymes and in situ effect of arsenite were analysed. Results Growth profile of mixed resident population of gut bacteria and pure isolates of Escherichia coli, Pseudomonas sp., Lactobacillus sp., and Staphylococcus sp. revealed an arsenite (2-20 ppm) concentration-dependent inhibition. The viability pattern of epithelial cells also showed similar changes. DHA and EA tests revealed significant inhibition (40%-72%) with arsenite exposure of 5 and 10 ppm in isolated gut bacteria and epithelial cells. Decrease in membrane alkaline phosphatase and Ca2 -Mg2 -ATPase activities was in the range of 33%-55% in four bacteria at the arsenite exposure of 10 ppm, whereas it was 60%-65% in intestinal epithelial villus cells. in situ incubation of arsenite using intestinal loops also showed more or less similar changes in membrane enzymes of resident gut bacterial population and epithelial cells. Conclusion The results indicate that facultative gut bacteria can be used as suitable in vitro model for the preliminary screening of arsenical gastrointestinal cytotoxic effects.
Keywords:Arsenic  Gut microflora  Intestinal bacteria  Intestinal epithelial cells  Membrane enzymes  GI-toxicity
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