益髓生血颗粒对血红蛋白H病患者抗氧化损伤和细胞因子水平的影响

目的 研究益髓生血颗粒对血红蛋白H病患者抗氧化损伤和造血相关细胞因子水平的影响。方法 采用安慰剂平行对照实验方法,选择30例血红蛋白H病患者,随机分为治疗组和对照组,每组15例,治疗组给予益髓生血颗粒进行治疗,对照组给予安慰剂进行治疗,疗程均为3个月。动态监测治疗前后患者Hb、RBC、HbH的水平;检测治疗前后血清及红细胞氧化与抗氧化损伤指标(SOD、MDA、GSH-PX)和细胞因子(SCF、GM-CSF、IL-3)活性。结果 自益髓生血颗粒治疗1~3个月,治疗组患者疗效性血液指标Hb水平与治疗前相比显著升高(P<0.01,P<0.05);RBC疗后1、3个月水平与疗前相比显著升高(P<0.05);Ret水平治疗后有升高的趋势。对照组经安慰剂治疗后血液指标Hb、RBC 、Ret水平与疗前相比均无明显改善。经益髓生血颗粒治疗3个月后,治疗组患者红细胞SOD、GSH-Px水平与疗前相比显著升高(P<0.01);MDA水平与疗前相比,显著降低(P<0.01);血清SOD水平与疗前相比显著升高(P<0.01);对照组各指标水平均无明显变化。经益髓生血颗粒治疗3个月后,治疗组患者SCF水平与疗前相比显著升高(P<0.05);GM-CSF、IL-3水平与疗前相比无明显变化;对照组各指标水平均无明显变化。结论 益髓生血颗粒治疗地中海贫血有良好的临床疗效,能显著改善患者血液参数水平。益髓生血颗粒的疗效生物学机制之一与提高HbH病患者抗氧化损伤能力和造血正调控细胞因子SCF活性有关。

Level of Anti-oxidative Damage and Cytokine in Patients of HbH Disease Treated by Yisui Shengxue Granule

Objective To study the influence of Yisui Shengxue granule on the levels of anti-oxidative damage and cytokine of patients with HbH disease. Methods A randomized single-blinde d trial was designed. Thirty patients with HbH disease were divided into two groups:15 patients in YSSXG-treated group and 15 in placebo parallel control group. The patients in the two groups were assigned to receive either YSSXG or placebo for three months.The blood parameters hemoglobin(Hb),red blood cell (RBC) and reticulocytes(Ret)] were detected dynamically.We also detected the SOD,MDA and GSH-PX from blood serum as well as red blood cell and the activity of SCF,GM-CSF and IL-3 from blood serum. Results After the treatment of Yisui Shengxue granule,the levels of blood parameter Hb of patients in the treatment group from the first to the end of months were still significantly higher than those of pre-treatment (P<0.01 or P<0.05) and the levels of RBC at the first and the third month were significantly higher than those of pre-treatment;the level of Ret had a rising tendency.The measurements of blood parameters in the placebo group had no statistically changing compared with those of pre-treatment.The activities of erythrocytic SOD and GSH-Px and the serum SOD were significantly higher than those of pre-treatment (P<0.01) and the activity of erythrocytic MDA was significantly lower than that of pre-treatment(P<0.01).The indicators of anti-oxidative damage of the placebo group were no remarkable changing.The level of SCF was significantly higer than that of pre-treatment (P<0.05)and the levels of GM-CSF and IL-3 had no significant difference with those of pre-treatment in the treatment group as well as in the placebo group. Conclusion Yisui Shengxue granule has a positive effect for the patients with HbH disease and dramatically improves the levels of blood parameters. One of biological mechanisms of curative effecacy was to increase the ability of anti-oxidative damage and positively regulate the activity of hematopoietic SCF.