祛风胜湿方对变应性鼻炎小鼠免疫球蛋白E及水通道蛋白5的影响

目的 观察祛风胜湿方对变应性鼻炎小鼠血清免疫球蛋白E(IgE)及水通道蛋白5(AQP5)的影响。方法 以卵清蛋白(OVA)+氢氧化铝基础致敏、局部以OVA激发复制变应性鼻炎小鼠模型,将其分为正常对照组、模型组、祛风胜湿方组、氯雷他定组。灌胃给药14天,观察各组对变应性鼻炎小鼠血清IgE、AQP5及鼻黏膜组织病理形态学的影响。结果 与正常对照组比较,模型组小鼠血清IgE及AQP5均显著增高(P<0.05);与模型组比较,各药物干预组的上述指标均显著降低(P<0.05)。结论 祛风胜湿方作用机制可能与其抑制IgE及AQP5表达有关。本实验为揭示中医"风能胜湿"与"祛风止痒"理论提供了实验数据支持。

Effects of Qufeng Shengshi Fang on the Behavior Index and AQP5 Expression in Allergic Rhinitis Mouse Model

Objective To observe the effects of Chinese medicine Qufeng Shengshi Fang (QSF) on serum immunoglobulin E (IgE) and aquaporin 5 (AQP5) in allergic rhinitis mouse model. Methods Allergic rhinitis (AR) mouse model was built by the basic stimulation of ovalbumin (OVA) and aluminum hydroxide with local excitation of OVA in mice. Animals were divided into four groups: normal control group, model group, QSF group and loratadine group. The effects of drugs on IgE, AQP5 and nasal mucosa pathological morphology was observed in different group after 14 days oral administration. Results Compared with the normal control group, levels of IgE and AQP5 were significantly increased in the model group (P<0.05), and they were significantly decreased in QSF and loratadine group than those in the model group (P<0.05). Conclusion The effects of QSF on AR mouse model might be related to the inhibition of IgE level and AQP5 expression. Also, the results may provide experimental data support for the theory of "Wind wins wet" and "Qufeng Zhiyang" in Chinese medicine.