高脂饲料喂养时间和STZ剂量对建立2型糖尿病大鼠模型的影响

目的探索高脂饲料喂养时间及STZ剂量对制备2型糖尿病大鼠模型及其血糖稳定性的作用。方法60只雄性sD大鼠随机分为6组：正常对照组（CN组）普通饲料喂养；模型1组（M1组）HFD喂养4周＋STZ35mg／（kg·bw）腹腔注射；模型2组（M2组）HFD喂养4周＋STZ38mg／（kg·bw）腹腔注射；模型3组（M3组）HFD喂养8周＋STZ25mg／（kg·bw）腹腔注射；模型4组（M4组）HFD喂养8周＋STZ30mg／（kg·bw）腹腔注射；模型5组（M5组）HFD喂养10周＋STZ25mg／（kg·bw）腹腔注射。STZ注射后观察大鼠空腹血糖（FBG）、空腹胰岛素（FINS）、甘油三酯（TG）、胆固醇（Tc）、口服糖耐量实验（OGTT）及血糖曲线下面积（AUC）变化。结果模型组FBG、FINS、TG、TC、OGTT各点血糖及AUC均显著高于正常对照组（P〈0．01）。随着高脂喂养时间和STZ注射剂量的增加2型糖尿病大鼠成模率和死亡率均呈上升趋势，M2组成模率高达到90％，但死亡率同样高达50％，M1和M4组成模率分别为70％和80％，死亡率低于M2。本研究各模型组2型糖尿病大鼠血糖在造模后第2周能够达到稳定状态。结论40％HFD喂养8周联合30mg／（kg·bw）STZ腹腔注射建立的2型糖尿病动物模型具有成模率高、死亡率低及血糖稳定等特点，是一种值得推广的造模方法。建议使用造模后第2周大鼠空腹血糖判断是否造模成功。

Effects of High - fat Diet Feeding Time and Streptozotocin Dose on Establishment of a Rat Model of Type 2 Diabetes Mellitus.

Objective To investgate the effects of different high - fatdiet feeding time and STZ doses on the establishment of a rat model of type 2 diabetes mellitus and the stability of blood sugar. Methods Sixty male SD rats were divided into 6 groups randomly：nor- mal controlgroup（NC） ; model 1 group（ M1 ）, high - fat diet（ four weeks） ＋ STZ35mg/（ kg ~ bw） intraperitoneal injection ; model 2 group （ M2 ）, high - fat diet （ four weeks） ＋ STZ38mg/（ kg · bw） ; model 3 groups （ M3 ）, high - fat diet （8 weeks） ＋ STZ25mg/（ kg · bw） ; model 4 group（M4） ,high - fat diet（8 weeks） ＋ STZ30mg/（ kg · bw） ; model 5 groups（MS） ,high - fat diet（ 10 weeks） ＋ STZ25mg/ （kg · bw）. We observed the fasting blood glucose（FBG） , fasting insulin（FINS） , triglycerides（TG） , cholesterol（TC） , oral glucose tol- erance test（OGTT） and glucose area under curve （AUC） of the rat model of type 2 diabetes after STZ injected. Results The FBG, FINS, TG, TC, blood glucose of OGTT and AUC of model groups were significantly higher than those of control group （ P 〈 0.01 ）. As the high -fat feeding time and the injection dose of STZ increasing,the type 2 diabetes model rates and mortality rates were all increased. M2 had the highestmodel rates and mortality rate, respectively as 90% and 50%. The model rats of M1 and M4 were respectively as 70% and 80% and compared with M2 the mortality rate was lower. The FBG of type 2 diabetes animal model in this study would reach a steady state in 2 weeks after STZ injection. Conclusion Forty percent HFD feeding 8 weeks with 30mg/（kg · bw） STZ is a kind of modeling method to be promoted. Type 2 diabetesanimal model established by this method had characteristics of high model rate, low mortality rate and a stable blood sugar. It is recommended to use the fasting glucose of 2 week after STZ injection to determine whether getting a successful model.