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三种方法转染绿色荧光蛋白质粒的效果评价
引用本文:孙丽娜、魏建春、张慧娟、张恩民、俞东征、海荣.三种方法转染绿色荧光蛋白质粒的效果评价[J].医学研究杂志,2010,39(1):27-29.
作者姓名:孙丽娜、魏建春、张慧娟、张恩民、俞东征、海荣
作者单位:中国疾病预防控制中心传染病预防控制所/传染病预防控制国家重点实验室,北京,102206
基金项目:科技部重大专项基金资助 
摘    要:目的比较常见的真核细胞转染技术,评价采用绿色荧光蛋白为报告基因来观察细胞转染效率高低的方法。方法 分别采用磷酸钙法、电穿孔法和脂质体法将pEGFP-C1质粒转染COS-7细胞,对每种方法的转染效率及对细胞的毒性进行评价。结果脂质体法的转染率为67%,电穿孔的转染率为43%,磷酸钙法的转染率为28%。脂质体法转染对细胞的毒性最小。结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中,脂质体法是效率高、安全性大的方法。

关 键 词:绿色荧光蛋白  转染  脂质体
收稿时间:2009/9/16 0:00:00
修稿时间:2009/12/3 0:00:00

Evaluation for Transfection Efficiency of GFP Report Gene in Three Different Ways
Sun Lin,Wei Jianchun,Zhang Huijuan,Zhang Enmin,Yu Dongzheng,Hai Rong.Evaluation for Transfection Efficiency of GFP Report Gene in Three Different Ways[J].Journal of Medical Research,2010,39(1):27-29.
Authors:Sun Lin  Wei Jianchun  Zhang Huijuan  Zhang Enmin  Yu Dongzheng  Hai Rong
Institution:Sun Lina,Wei Jianchun,Zhang Huijuan,Zhang Enmin,Yu Dongzheng,Hai Rong.State Key Laboratory for Infectious Disease Control , Prevention,Institute for Communicable Disease Control , Prevention,Chinese Center for Disease Control , Prevention,Beijing 102206,China
Abstract:Objective To evaluate the efficiency for eukaryotic cell transfection by using GFP as a report gene in common transfection ways. Methods COS-7 cell was transfected with pEGFP-C1 by calcium acid phosphate,electroporation and lipofectamine respectively. The transfection efficiency and cell survival rate were evaluated. Results The transfection efficiency was 67%,43% and 28% by lipofectamine,electroporatin and calcium acid phosphate. Most cells survived by transfection with lipofectamine. Conclusion Using GFP ...
Keywords:GFP  Transfection  Iiposome  
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