过表达 mimecan对人脐静脉内皮细胞增殖和凋亡的作用初探

目的 应用反转录病毒载体技术构建稳定过表达mimecan的人脐静脉内皮细胞株（HUVEC）,并观察过表达mime-can后HUVEC增殖及凋亡的变化.方法 应用反转录病毒载体构建稳定过表达mimecan的HUVEC细胞株,real-time PCR和Western blot法检测mRNA和蛋白水平的表达.应用CCK-8方法检测过表达mimecan后HUVEC增殖的变化,应用Annexin V-PE染色观察过表达mimecan后HUVEC凋亡的变化.结果 构建了稳定过表达mimecan的HUVEC细胞株.过表达mimecan后,HUVEC增殖能力降低,凋亡率增加.结论 过表达mimecan抑制HUVEC的增殖,促进其凋亡.

Over-expression of Mimecan in Human Umbilical Vein Endothelial Ceil Affects Cell Proliferation and Apoptosis

Objective To construct a cell model stably over-expressing mimecan in human umbilical vein endothelial cell （HUVEC）,and observe the effect of mimecan on HUVEC proliferation and apoptosis.Methods In vitro,human mimecan was stably over-expressed in HUVEC using a retrovirus system.Human mimecan expression was verified by quantitative real-time PCR and western blot analysis.The proliferation of HUVEC transduction of mimecan was measured by CCK-8 assay.By AnnexinV-PE of staining phosphatidyl serine,we examined the apoptosis rate of HUVEC after over-expression of mimecan.Results In our study,we successfully established a cell model stably over-expressing mimecan in HUVEC using retrovirus system.And,real-rime PCR and western blot revealed the over-expression of mimecan in HUVEC.Through CCK-8 assay,it was found that the proliferation rate of HUVEC transduction of mimecan was lower compared to control cell.By Annexin V-PE of staining phosphatidyl serine,over-expression of mimecan induced HUVEC apoptosis.Conclusion These data indicate that mimecan may involve in the pathogenesis of atherosclerosis through affect the proliferation and apoptosis of HUVEC.