青藤碱对人结肠癌细胞株SW480增殖和细胞周期的影响

目的 研究青藤碱对人结肠癌SW480细胞增殖和细胞周期的影响.方法 用不同浓度的青藤碱处理体外培养的人结肠癌SW480细胞24、48、72h后,采用CCK法检测细胞的增殖活性,采用流式细胞术检测细胞的细胞周期分布,并用光镜和透射电镜观察SW480细胞的形态学变化.结果 青藤碱在体外可抑制SW480细胞的增殖,并呈时间和剂量依赖性.干预48h后,中、低浓度的青藤碱（8mmol/L、4mmol/L）可诱导SW480细胞G1期细胞比例增加,而高浓度的SIN（16mmol/L、10mmol/L）可诱导G1期、G2期细胞比例增加.光镜与电镜下均见凋亡细胞增多.结论 青藤碱在体外能抑制人结肠癌SW480细胞增殖和诱导该细胞系的凋亡,其机制可能与其阻滞细胞周期进程有关.

Effect of Sinomenine on Cell Proliferation and Cell Cycle in Human Colon Cancer SW480 Cells

Objective To investigate the effect of sinomenine on cell proliferation and cell cycle progression in human colon cancer SW480 cells.Methods By technique of cell culture in vitro,SW480 cells were treated with sinomenine in different concentrations for 24,48 and 72h,then cell viability of each group was measured by cell counting Kit-8.Cell cycle was analyzed by flow cytometry.The morphological features of cell apotosis were observed under light and transmission electron microscope.Results Sinomenine could inhibit proliferation of SW480 cells in a time-and dose-depengdent manner in vitro.Flow cytometric analysis showed a Gl arrest at medium and low concentration（8mmol/L,4mmol/L）,but both G1 and G2 arrest at high concentration （16mmol/L,10mmol/L） after 48h exposure.The occurrence of cell apoptosis can be detected under light and transmission electron microscope.Conclusion Sinomenine could inhabit proliferation and induce apoptosis of SW480 cells in vitro.Its mechanism may be relevent to cell cycle arrest.