RNA干扰HO-1的表达增强食管鳞癌Eca109细胞对氟尿嘧啶化疗的敏感度

目的 探讨小干扰RNA沉默HO-1基因表达后食管鳞癌Eca109细胞对氟尿嘧啶化疗敏感度的影响.方法 利用HO-1小干扰RNA（siRNA）及氯化高铁血红素分别沉默和诱导食管鳞癌Eca109细胞HO-1基因的表达,RT-PCR检测HO-1 mRNA水平,Western blot法检测HO-1蛋白表达水平,分别应用MTS法、流式细胞仪检测干预后Eca109细胞对氟尿嘧啶敏感度的变化.结果 RT-PCR、Western blot法检测结果显示,HO-1 siRNA能够有效抑制食管鳞癌Eca109细胞HO-1的表达.HO-1基因干扰后,氟尿嘧啶对Eca109细胞增殖抑制作用增强、凋亡细胞比例增加.结论 干扰HO-1的表达可以显著增强食管鳞癌Eca109细胞对氟尿嘧啶化疗的敏感度.

Interference of Heme Oxygenase-1 Gene Expression Enhances the Sensitivity of Esophageal Squamous Cell Carcinoma Eca109 to Fluorouracil

Objective To explore the effect of sensitivity of esophageal carcinoma cell line Eca109 to fluorouracil by RNA interference suppressing the expression of HO-1.Methods The small interfering RNA （siRNA） targeting HO-1 gene was designed and synthesized to suppress the gene expression of HO-1,and hemin was used to promote the expression of HO-1.The mRNA and protein expression levels of HO-1 were evaluated by RT-PCR and Western blot.The effect of sensitivity of esophageal carcinoma cell line Eca109 to Fluorouracil was detected by MTS and flow cytometry.Results RT-PCR and Western blot test results showed that Hemin（10μmol/L） could effectively induce the expression of HO-1,while HO-1 siRNA could successfully inhibit the expression of HO-1 in esophageal squamous cell line Eca109.The proliferative capacity was markedly decreased and apoptosis index significantly increased after transfection with HO-1 siRNA.Conclusion The sensitivity of esophageal squamous cell line Eca109 to fluorouracil could be significantly improved by RNA interference suppressing the expression of HO-1.