脑出血患者抵抗素基因+299G>A位点多态性分析

目的揭示浙江地区汉族脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素及C-反应蛋白水平的相关性。方法选取344例高血压性基核出血患者和344例年龄及性别均配对的健康体检者,入院时或体检时抽取外周血提取DNA,采用聚合酶链反应-限制性片段多态性分析基因型,同时采用ELISA法检测脑出血患者血浆抵抗素及C-反应蛋白浓度,统计分析其相关性。结果酶切后可见抵抗素基因+299G>A位点有GG、GA和AA 3种基因型。健康体检者等位基因G和A的频率分别为64.8%和35.2%,基因型GG、GA和AA的频率依次为41.9%、45.9%和12.2%。脑出血患者等位基因G和A的频率分别为69.3%和30.7%,基因型GG、GA和AA的频率依次为为49.1%、40.4%和10.5%。经Hardy-Weinberg遗传平衡定律检验,脑出血患者及健康体检者各基因型频率均符合遗传平衡。经卡方检验,脑出血患者及健康体检者各基因型差异无统计学意义(P=0.159)。GG、GA和AA基因型脑出血患者血浆抵抗素浓度依次为(23.9±7.2)ng/ml、(26.4±7.2)ng/ml和(26.9±9.7)ng/ml,血浆C-反应蛋白浓度依次为(7.5±2.2)mg/L、(8.3±2.7)mg/L和(8.4±3.0)mg/L。采用方差检验,GG基因型患者血浆抵抗素和C-反应蛋白浓度均显著低于GA和AA基因型患者(P均<0.05),而GA和AA基因型患者血浆抵抗素和C-反应蛋白浓度差异无统计学意义(P均>0.05)。采用多元线性回归分析,脑出血患者入院时血浆抵抗素浓度与C-反应蛋白浓度显著正相关(t=7.032,P<0.001)。结论浙江地区汉族脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素和C-反应蛋白浓度显著相关,可能参与脑出血炎症反应。

+299G>A Polymorphism of Resistin Gene in the Patients with Intracerebral Hemorrhage

Objective This study aimed to examine the relations of the +299G>A single nucleotide polymorphism of resistin gene to the plasma resistin and C-reactive protein concentrations in intracerebral hemorrhage.Methods Totally 344 Chinese Han patients with intracerebral hemorrhage and 344 age-and sex-matched healthy subjects were included in this study.Plasma resistin and C-reactive concentrations were measured in the patients,as well as +299G>A polymorphisms of resistin gene in the healthy subjects and patients were genotyped using PCR-restriction fragment length polymorphism.Results The genotypes of the +299G>A polymorphism of resistin gene included GG,GA and AA.The G and A allele frequencies of the healthy subjects were 64.8% and 35.2%,respectively.The genotype GG,GA and AA frequencies of the healthy subjects were 41.9%,45.9% and 12.2%,respectively.The G and A allele frequencies of the patients were 69.3% and 30.7%,respectively.The genotype GG,GA and AA frequencies of the patients were 49.1%,40.4% and 10.5%,respectively.The genotype frequencies of the +299G>A polymorphism of resistin gene in the healthy subjects and patients were in the Hardy-Weinberg equilibrium.The genotype frequencies between healthy subjects and patients showed no significant differences(P=0.159).Plasma resistin levels of the patients with the genotype GG,GA and AA were(23.9±7.2)ng/ml,(26.4±7.2)ng/ml and(26.9±9.7)ng/ml respectively,as well as their C-reactive protein levels were(7.5±2.2)mg/L,(8.3±2.7)mg/L and(8.4±3.0)mg/L respectively.By analysis of variance,the common homozygote(GG) had the lowest resistin and C-reactive protein plasma concentrations(P all <0.05);the plasma resistin and C-reactive protein concentrations between heterozygote(GA) and rare allele homozygote(AA) did not differ significantly(P all >0.05).Plasma resistin level was significantly associated with plasma C-reactive protein level(t=7.032,P<0.001) using multivariate linear regression.Conclusion Our results implied that the +299G>A single nucleotide polymorphism of resistin gene was associated with plasma resistin and C-reactive protein concentrations and seemed to be potentially involved in the inflammatory component of intracerebral hemorrhage.