| miR-221和miR-222在急性髓细胞白血病初发患者中的表达研究 |
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| 引用本文： | 李宝青,薛向阳,夏轶姿,朱宝玲,何牧卿,李向阳,张丽芳.miR-221和miR-222在急性髓细胞白血病初发患者中的表达研究[J].医学研究杂志,2011,40(6):110-113. |
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| 作者姓名： | 李宝青 薛向阳 夏轶姿 朱宝玲 何牧卿 李向阳 张丽芳 |
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| 作者单位： | 1. 温州医学院附属第二医院,325000
2. 温州医学院微生物教研室,325000 |
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| 摘 要： | 目的探讨miR-221和miR-222在急性髓细胞白血病(AML)骨髓有核细胞中的表达及意义。方法选择16例AML初发患者和44例非白血病患者骨髓标本,分离有核细胞,抽提总RNA,以U6为内参,采用茎环Realtime RT-PCR法检测并比较AML和非白血病患者骨髓有核细胞中miR-221和miR-222的表达,同时比较这两种miRNAs在AML中M3、M4和M5三种亚型间的表达水平。结果 miR-221和miR-222在AML初诊患者骨髓中相对表达量(N=2-ΔCt)明显高于非白血病患者组(P<0.05);这两种miRNAs在M5型AML患者表达最高,但在AML三种亚型间表达水平未见显著的统计学意义(P>0.05)。结论 miR-221和miR-222有可能在为AML的诊断、治疗和预后上,提供一个新的标准和靶点指标。
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| 关 键 词： | miR-221 miR-222 急性髓细胞白血病 |
| 收稿时间： | 2010/11/14 0:00:00 |
The Expression and Significance of miR-221 and miR-222 in the Acute Myeloid Leukemia |
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| Li Baoqing,Xue Xiangyang,Xia Yizi,et al..The Expression and Significance of miR-221 and miR-222 in the Acute Myeloid Leukemia[J].Journal of Medical Research,2011,40(6):110-113. |
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| Authors: | Li Baoqing Xue Xiangyang Xia Yizi |
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| Institution: | Li Baoqing,Xue Xiangyang,Xia Yizi,et al.The Second Affiliated Hospital of Wenzhou Medical College,Department of Microbiology and Immunology,Wenzhou Medical College,Zhejiang 325027,China |
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| Abstract: | Objective To investigate the expression and clinical significance of miR-221 and miR-222 in the marrow of acute myeloid leukemia(AML).Methods Marrow tissues from 16 newly diagnosed AML patients and 44 non-leukemia(NL) patients were collected and mononucleated cell were separated.Total RNA from nucleated cells of these samples were extracted and analyzed for miR-221 and miR-222 expression by stem-loop real-time RT-PCR.The differences in subtypes M3,M4 and M5 patients and the change of miR-221 and miR-222 exp... |
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| Keywords: | miR-221 miR-222 |
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