胰岛素样生长因子-1促进脂肪干细胞迁移能力的研究

目的 探讨胰岛素样生长因子-1（insulin-like growth factor-1，IGF-1）对脂肪间充质干细胞（adipose-derived stem cells，ADSCs）迁移能力的影响，为勃起功能障碍的治疗建立实验基础。方法 采用酶消化法分离提取SD大鼠皮下脂肪干细胞，以含有10%的胎牛血清的DMEM培养基培养。通过流式细胞仪检测ADSCs表面标志物（CD34、CD44、CD45）的表达情况。采用终浓度为20ng/ml IGF-1预处理ADSCs，观察IGF-1对ADSCs迁移能力以及CXCR4蛋白的表达情况。结果 流式细胞术显示ADSCs的表型分子CD44呈阳性，CD34、CD45呈阴性。IGF-1可上调CXCR4的表达，并且促进 ADSCs向SDF-1迁移。结论 本实验成功分离培养ADSCs，IGF-1能够提高ADSCs表面CXCR4蛋白的表达，从而促进ADSCs迁移，并为下一步将IGF-1预处理的ADSCs应用于大鼠体内勃起功能障碍的治疗提供基础。

Insulin-like Growth Factor 1 Enhances the Migratory Capacity of Adipose-derived Stem Cells

Objective To explore the effects of insulin-like growth factor-1 (IGF-1) on migration of adipose derived stem cells (adipose-derived stem cells, ADSCs),and to provide experimental basis for the treatment of erectile dysfunction. Methods ADSCs were obtained from Sprague-Dawley rats by enzyme digestion,which was cultured with DMEM with 10% fetal bovine serum.Flow cytometry was adopted to analyze the expression of surface markers (CD34, CD44,CD45).The final concentration of 20ng/ml IGF-1 pretreatment ADSCs,observe the migration ability and CXCR4 protein expression of ADSCs. Results CD44 of ADSCs were positively expressed and CD34, CD45 were negatively expressed. The expression of CXCR4 on ADSCs and migration of MSCs were increased by IGF-1. Conclusion ADSCs of SD rats were isolated and cultured successfully. IGF-1 had promotion effects on the CXCR4 protein expression of ADSCs surface and the migration of ADSCs, which provided basis for the treatment of erectile dysfunction in rats with the application of the ADSCs of IGF-1 pretreatment in the next step.