高血糖对成骨细胞增殖分化影响的实验研究

目的观察糖尿病大鼠股骨成骨细胞的病理改变以及高糖对成骨细胞增殖分化能力的影响,为临床治疗糖尿病骨质疏松症提供一定的实验基础。方法 2月龄雄性SD大鼠20只,随机分正常对照组和糖尿病骨质疏松组(n=10),糖尿病组大鼠经腹腔注射链脲佐菌素造模,同时体外分离培养成骨细胞,随机分为正常培养基组和高糖培养基组。成模6周麻醉后处死大鼠,分离各组大鼠的左侧股骨,于小动物放射成像系统上,测其近段骨密度,HE染色右侧股骨,观察比较两组大鼠股骨成骨细胞、破骨细胞的形态。同时两组成骨细胞各自培养48h后检测其形态及ALP阳性率。结果糖尿病组大鼠股骨骨密度显著低于对照组,同时骨皮质下成骨细胞显著减少,骨细胞呈扁平或星形,少见新骨形成。而高糖培养组成骨细胞其碱性磷酸酶(ALP)阳性率明显低于正常培养组,增殖分化能力减弱。结论高血糖状态下成骨细胞减少及增殖分化能力减弱,造成成骨细胞和破骨细胞的失衡,从而影响了骨组织的代谢,可能是造成糖尿病骨质疏松症的一个原因。

Experimental Study of Effects of Hyperglycaemia on Osteoblast Proliferation and Differentiation

Objective To study the osteoblasts pathological changes in femur bone and study the effect of hyperghycaemia on proliferation and differentiation of osteoblasts,providing experimental basis for the clinical treatment of diabetic osteoporosis.Methods Two months old male SD 20 rats were randomly divided into normal control group and diabetic osteoporosis group(n=10).Diabetic rats were induced by intraperitoneal injection of streptozotocin,while osteoblasts cultured in vitro,and were divided into normal medium group and high glucose medium group.After 6 weeks,the rats were anesthesia and left femur was isolated.The bone mineral density of proximal were measured by small animal radiography systems.The pathological changes of osteoblasts and osteoclasts were observed by HE staining.The morphology and ALP-positive rate of osteoblasts in two groups were detected after 48h culture.Results The bone mineral density in diabetic group was significantly lower than that of the control group,while the osteoblasts under cortical bone were significant reduced,bone cells were flat or star,and rare new bone formation was seen.The alkaline phosphatase(ALP) of osteoblasts cultured in high glucose were significantly lower than the normal culture group.Proliferation and differentiation were also weakened.Conclusion Under hyperglycaemia condition,the osteoblast proliferation and differentiation was reduced and weaken,resulting in osteoblasts and osteoclasts imbalance,which effects the metabolism of bone tissue,and may be the cause of diabetic osteoporosis.