预防性星状神经节阻滞对慢性应激抑郁大鼠海马神经元凋亡的影响

目的 研究星状神经节阻滞对慢性应激抑郁大鼠行为学及凋亡蛋白Bcl-2和Bax表达的影响.方法 SD雄性大鼠（n=32）随机分为4组,每组8只：对照＋盐水（CC组）、对照＋星状神经节阻滞（CS组）、慢性应激＋盐水（SC组）、慢性应激＋星状神经节阻滞（SS组）.采用不可预测的慢性应激致抑郁模型,用布比卡因行星状神经节阻滞干预.所有动物观察21天后测量体重变化,行糖水实验.放血处死后,留取脑组织,采用Western blot法检测海马组织Bcl-2、Bax的表达.结果 21天的慢性应激后,应激组的大鼠体重增长明显低于对照组大鼠（P＜0.05）,糖水摄取量也低于对照组（P＜0.05）.应用星状神经节阻滞干预后,上述指标均明显改善（P＜0.05）.在Western blot法检测中,应激组大鼠海马中Bcl-2的表达明显低于对照组,而经星状神经节阻滞治疗后,大鼠蛋白表达含量有所增加（P＜0.05）.Bax的表达在应激组中明显升高,但在星状神经节阻滞干预后,蛋白表达水平有所下降（P＜0.05）.结论 星状神经节阻滞可改善慢性应激抑郁行为,其机制可能是抑制凋亡蛋白Bax的表达,促进抗凋亡蛋白Bcl-2的表达,减少海马神经元的凋亡.

Effect of Stellate Ganglion Block on Apoptosis of the Hippocampal Neurons in Depression Rats Induced by Chronic Mild Stress

Objective To investigate whether stellate ganglion block can reverses behavior and the expression of Bcl-2 and Bax in rat model of chronic mild stress.Methods After one week habituation,32 SD rats were randomly divided into 4 groups（n =8）：control plus daily saline in stellate ganglion group （CC）,control plus daily SGB group （CS）,chronic mild stress （CMS） model plus daily saline in stellate ganglion group （SC）,and CMS model plus daily SGB group （SS）.The animals were subjected to a chronic mild stress procedure for a period of three consecutive weeks,and stellate ganglion block was performed.At the end of experiment,the change of weight was recorded,and then rats were subjected to sucrose preference test.The expression of Bcl-2 and Bax was detected by Western blot.Results Rats exposed to chronic mild stress gained weight more slowly than controls during the 21 days of observation （P ＜ 0.05）.The rats in SS group gained more weight than the rats in SC group （P ＜ 0.05）.Compared with CC group,sucrose solution consumption in SC group decreased significantly （P ＜ 0.05）.After stellate ganglion block treatment,sucrose solution consumption in SS group were significantly higher than SC group （P ＜0.05）.According to Western-blot method,the expression of Bcl-2 in group SC was significantly lower than group CC,but the expression of Bax in group SC was significantly higher than group CC （P ＜ 0.05）.At the end of experiment,stellate ganglion block treatment significantly increased levels of Bcl-2 expression（P ＜ 0.05）,and decreased levels of Bax expression in hippocampus （P ＜ 0.05）.Conclusion Stellate ganglion block could play a role of antidepressant by reducing apoptosis of hippocampal neurons.