| 端粒酶永生化的人单克隆神经前体细胞系诱导表达多巴胺D2受体 |
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| 引用本文： | 扈启宽,林昌盛,白云,白洁,何其华,沈丽,李凌松.端粒酶永生化的人单克隆神经前体细胞系诱导表达多巴胺D2受体[J].北京大学学报(医学版),2003,35(3):271-273. |
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| 作者姓名： | 扈启宽 林昌盛 白云 白洁 何其华 沈丽 李凌松 |
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| 作者单位： | 1. 北京大学医学部干细胞中心,北京,100083
2. 广州暨南大学生物工程研究所 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);2001CB510100; |
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| 摘 要： | 目的:检测人神经前体细胞系HNG1210-E能否诱导表达多巴胺D2受体mRNA和受体蛋白,表达的受体是否有生理功能.方法:细胞因子诱导分化,RT-PCR和免疫荧光染色检测D2的表达; Fluo-3 测定多巴胺刺激产生的胞浆Ca2+.结果:RT-PCR显示D2 mRNA诱导后阳性,免疫荧光结果显示诱导后D2受体蛋白在细胞膜表面阳性,Fluo3法测定胞浆Ca2+,结果显示诱导后的细胞对5 mmol*L-1多巴胺递质反应使胞浆Ca2+快速上升.结论:从mRNA、蛋白和生理功能3个层次均证明HNG1210-E细胞系诱导后表达D2.
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| 关 键 词： | 神经前体细胞 端粒酶永生化 多巴胺D2受体 细胞分化 |
| 文章编号： | 1671-167X(2003)03-0271-03 |
Induced expression of dopamine D2 receptor in human neuro-progenitor cell line HNG1210-E |
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| Qikuan Hu,Changsheng Lin,Yun Bai,Jie Bai,Qihua He,Li Shen,Lingsong Li.Induced expression of dopamine D2 receptor in human neuro-progenitor cell line HNG1210-E[J].Journal of Peking University:Health Sciences,2003,35(3):271-273. |
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| Authors: | Qikuan Hu Changsheng Lin Yun Bai Jie Bai Qihua He Li Shen Lingsong Li |
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| Institution: | Peking University Stem Cell Research Center, Beijing 100083, China. |
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| Abstract: | OBJECTIVE: To investigate whether the dopamine receptor D2 can express or can be induced to express in HNG1210-E, a monoclonal, telomerase-immortalized, human neural progenitor cell line. METHODS: By means of RT-PCR, immuno-fluorescent staining, and fluo3 Ca2+ imaging to the expression of D2 mRNA and protein as well as the reaction to dopamine were demonstrated. RESULTS: HNG1210-E cells could be induced to express D2 mRNA and its proteins. The induced cells also reacted to dopamine (5 mmol.L-1), which caused rapid rising of cytoplasm Ca2+. CONCLUSION: HNG1210-E cells can be induced to express D2 mRNA and functional proteins. |
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| Keywords: | Neural progenitor cell Receptor dopamine D 2 Cell differentiation |
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