| 慢性根尖周炎患牙根尖外表面的细菌生物膜 |
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| 引用本文： | 郭惠杰,王嘉德.慢性根尖周炎患牙根尖外表面的细菌生物膜[J].北京大学学报(医学版),2009,41(5). |
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| 作者姓名： | 郭惠杰 王嘉德 |
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| 作者单位： | 北京大学人民医院口腔科,北京,100044;北京大学口腔医院牙体牙髓科 |
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| 摘 要： | 目的:对慢性根尖周炎患牙根尖外表面的生物膜进行细菌学研究和形态学观察,了解生物膜中细菌的种类、组成及其超微结构.方法:选择临床诊断为慢性根尖周炎且不能保留的患牙10例,包括未经治疗及根管治疗失败的患牙各5例,同时将5例因正畸减数拔除的健康前磨牙作为对照组.所有样本的牙冠部无菌消毒后进行根管内取样、接种、厌氧培养后检测根管内的细菌种类.息牙拔除即刻3%(体积分数)戊二醛固定,取距根尖5 mm的牙根经梯度脱水、临界点干燥、喷金处理后进行扫描电镜观察.结果:10例慢性根尖周炎患牙拔除前根管内细菌培养均为阳性,其中微小消化链球菌和具核梭杆菌检出率较高.在5例未治疗的慢性根尖周炎患牙中仅有1例根尖外表面存在生物膜结构,主要由球菌组成;而在5例根管治疗失败的根尖周炎患牙的根尖外表面均可观察到浓密的细菌膜状结构,由球菌、杆菌及/或丝状菌组成,并且在超填的牙胶尖表面还观察到由球菌和丝状菌组成的典型的"玉米棒芯"样结构.对照组中则未有细菌存在.结论:慢性根尖周炎患牙的根尖外表面存在细菌生物膜结构,该生物膜的存在与临床上难治性慢性根尖周炎密切相关.
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| 关 键 词： | 根尖周炎 细菌感染 生物膜 根管疗法 牙根尖 |
Bacterial biofilm on the apical external root surfaces of human teeth associated with chronic periradicular lesions |
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| GUO Hui-jie,WANG Jia-de.Bacterial biofilm on the apical external root surfaces of human teeth associated with chronic periradicular lesions[J].Journal of Peking University:Health Sciences,2009,41(5). |
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| Authors: | GUO Hui-jie WANG Jia-de |
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| Institution: | GUO Hui-jie1,WANG Jia-de2(1.Department of Stomatology,Peking University People's Hospital,Beijing 100044,China,2.Department of Operative Dentistry , Endodontics,Peking University School , Hospital of Stomatology) |
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| Abstract: | Objective:To investigate the patterns of microbial infection on the apical external root surfaces of treated and untreated teeth associated with chronic apical periodontitis and to study bacteria in the biofilm in order to find out the species,constitution and origination of bacteria in periapical biofilm.Methods: Ten teeth with chronic apical periodontitis from patients of the Department of Stomatology of People's Hospital,Peking University: 5 untreated teeth with a radiographically visible chronic perirad... |
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| Keywords: | Periapical periodontitis Bacterial infections Biofilms Root canal therapy Tooth apex |
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