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不同成熟期人卵母细胞慢速冷冻的初步研究
引用本文:陈子江,李梅,马金龙,李媛,马水英,高选.不同成熟期人卵母细胞慢速冷冻的初步研究[J].北京大学学报(医学版),2004,36(6):571-574.
作者姓名:陈子江  李梅  马金龙  李媛  马水英  高选
作者单位:1. 山东大学山东省立医院生殖医学中心,济南,250021
2. 山东大学山东省立医院病理科,济南,250021
基金项目:国家自然科学基金 , 高等学校博士学科点专项科研项目
摘    要:目的:研究不同成熟期人卵母细胞冻融后超微结构的改变和冷冻保护剂浓度对卵母细胞发育潜能的影响.方法:收集多囊卵巢综合征患者辅助生殖周期获得的卵母细胞进行慢速冷冻.根据冷冻保护剂中蔗糖浓度的不同(0.1,0.2或0.3 mol/L)对不同成熟期的卵母细胞分组,进行发育潜能的实验研究,同时收集部分未冷冻的和冻融后的卵母细胞进行超微结构观察.并将部分冷冻卵母细胞获得的胚胎用于临床患者.结果:0.2 mol/L的蔗糖浓度较0.1 mol/L更有利于各成熟期卵母细胞的冷冻,成熟卵母细胞用0.3 mol/L的蔗糖冷冻效果较好,经体外受精得到的胚胎移植后,获得2例临床妊娠.电镜结果显示,未成熟卵母细胞卵浆内的线粒体和泡状物较成熟卵母细胞少,且分布没有规律;冷冻对成熟卵母细胞超微结构的影响较未成熟卵母细胞大.结论:适当提高冷冻保护剂中蔗糖浓度,有利于各成熟期卵母细胞的冷冻保存,0.3 mol/L浓度的蔗糖冷冻保护剂是成熟卵母细胞冷冻的最佳浓度.慢速冷冻可以引起卵母细胞超微结构的改变.

关 键 词:卵母细胞/超微结构  低温保存  受精  体外  不同成熟期  未成熟卵母细胞  慢速冷冻  研究  maturity  different  oocytes  human  最佳  细胞冷冻  冷冻保存  细胞超微结构  规律  分布  线粒体  卵浆  显示  电镜  临床妊娠  胚胎移植
文章编号:1671-167X(2004)06-0571-04
修稿时间:2004年9月20日

The cryopreservation of human oocytes at different maturity stages
CHEN Zi-jiang.The cryopreservation of human oocytes at different maturity stages[J].Journal of Peking University:Health Sciences,2004,36(6):571-574.
Authors:CHEN Zi-jiang
Institution:Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, China. zjchen59@126.com
Abstract:Objective:To examine the ultrastructure changes and the effects ot different sucrose con-centrations on the developmental potential of human frozen-thawed oocytes at different maturity stages.Methods:Oocytes at different maturity stages collected from polycystic ovarian syndrome patients wereinvolved in the study.Different sucrose concentrations(0.1,0.2 or 0.3 mol/L)were used to study thedevelopmental potential of the frozen-thawed oocytes.Non-frozen and frozen-thawed different maturity oo-cytes were processed for transmission electron microscopy observation.Results:The results revealed thatthe mitochondria and the vesicles in the immature oocytes cytoplasm were fewer than those in the matureoocytes without regular distribution.Electron density of the mitochon dria and distribution of the vesiclesin the mature oocytes changed with the cryopreservation.No remarkable change was produced in the im-mature oocytes between non-frozen and frozen-thawed oocytes.Cryopreserving with 0.2 mol/L sucrose re-sulted in perfect development potential for both mature and immature oocytes than that with 0.1 mol/L.Study involving 0.3 mol/L sucrose in the cryoprotectant resulted in higher survival rate and clinical preg-nancies.Conclusion:The results suggest that sucrose concentration of 0.3 mol/L in the cryoprotectantsolution is efficient in freezing oocytes with slow-freezing method.Cryopreservation with slow-freezingmethod could produce ultrastructure changes in the human oocytes.
Keywords:Oocytes/ultrastruct  Cryopreservation  Fertilization  in vitro
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