| 用nested RT-PCR法检测肝癌患者外周血癌细胞 |
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| 引用本文： | 刘宇慧,周柔丽,芮静安.用nested RT-PCR法检测肝癌患者外周血癌细胞[J].北京大学学报(医学版),1997(3). |
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| 作者姓名： | 刘宇慧 周柔丽 芮静安 |
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| 作者单位： | 北京医科大学细胞生物学教研室!100083(刘宇慧，周柔丽)，北京邮电总医院肿癌研究所(芮静安) |
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| 摘 要： | 目的：寻求一种高灵敏的检测肝癌患者外周血中癌细胞的方法。方法：采用nestedRT－PCR法检测外周血中的肝癌细胞。结果：能从2ml人外周静脉血中检测到10个左右肝癌细胞表达的人甲胎蛋白mRNA。结论：此方法特异性强，灵敏度高，可作为一种理想的临床检测手段。
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| 关 键 词： | 聚合酶链反应 甲胎蛋白类 肝肿瘤／诊断 肿瘤循环细胞／遗传学 |
DETECTION OF HEPATOMA CELLS IN PERIPHERAL BLOOD OF THE PATIENTS WITH HCC WITH NESTED RT-PCR |
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| LIU Yuhui, ZHOU Rouli, RUI Jingan.DETECTION OF HEPATOMA CELLS IN PERIPHERAL BLOOD OF THE PATIENTS WITH HCC WITH NESTED RT-PCR[J].Journal of Peking University:Health Sciences,1997(3). |
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| Authors: | LIU Yuhui ZHOU Rouli RUI Jingan |
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| Abstract: | bjective:The experiment aimed at finding a highly sensitive assay for detection of hepatoma cells in peripheral blood of the patients with HCC. Methods: Using nested RT-PCR method to detect hepatoma cells in peripheral blood.Results: The AFP mRNA contained in ten hepatoma cells was detected from 2 ml peripheral blood. ConcIuslon: The results suggested that the highly sensitive assay for the AFP mRNA expressed in cancer cells in peripheral blood might be a valuable method for clinical diagnosis of HCC. |
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| Keywords: | Alpha-Fetoproteins Liver neoplasms/diag Polymerase chain reaction Neoplasms circulating cells/genet |
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