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周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响
引用本文:张丁,李小彤,傅民魁.周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响[J].北京大学学报(医学版),2001,33(2):118-121.
作者姓名:张丁  李小彤  傅民魁
作者单位:^A北京大学口腔医院正畸科,北京 100081^B北京大学医学部口腔医学院(口腔医院)^C73259^D1%^A北京大学口腔医院正畸科,^B北京大学医学部口腔医学院(口腔医院)^C73259^D2
基金项目:国家自然科学基金;30070821,39200139;
摘    要:目的:了解机械力介导的牙周膜细胞中成骨样细胞特性的改变,以利于深入研究正畸牙齿移动的机制。方法:应用地高辛标记寡核苷酸探针进行细胞原位杂交,检测在间歇性机械牵张力作用下人牙周膜细胞中的成骨样细胞表型蛋白碱性磷酸酶和骨钙素在mRNA转录水平的表达改变。结果:间歇性牵张力作用增强人牙周膜细胞碱性磷酸酶和骨钙素基因的表达。结论:间歇性牵张力作用诱导人牙周膜细胞向成骨样细胞的分化。

关 键 词:牙周组织  成骨细胞  碱性磷酸酶/代谢  骨钙素/代谢  间歇性牵张力  RNA  信使/代谢  
文章编号:1000-1530(2001)02-0118-04

Effect of cyclical mechanical force on expression of mRNAs for osteoblast-like marker proteins in human periodontal ligament cells in vitro
ZHANG Ding,LI Xiao-tong,FU Min-kui.Effect of cyclical mechanical force on expression of mRNAs for osteoblast-like marker proteins in human periodontal ligament cells in vitro[J].Journal of Peking University:Health Sciences,2001,33(2):118-121.
Authors:ZHANG Ding  LI Xiao-tong  FU Min-kui
Abstract:Objective: To study the role of mechanical stress on osteoblast-like characteristics expressed by human periodontal ligament cells in vitro. Methods: Human periodontal ligament cells (hPDLC) were cultured by explant method. We investigated the gene expression for osteoblast-like marker proteins in periodontal ligament stimulated by cyclical mechanical force in vitro by in situ hybridization with non-radioisotopic oligonucleotide probe for alkline phosphatase (ALP) and osteocalcin (OCN). Results: Human periodontal ligament cells exhibited a stronger signal for ALP mRNA stimulated by cyclical mechanical force, and exhibited a moderately intense positive signal for OCN mRNA—OCN was a marker of differentiated osteoblast-like cells. Conclusion: These findings suggest that mechanical force plays role in differentiation from hPDLC to osteoblast-like cells.
Keywords:Periodontium  Osteblast  Alkaline phosphatase/metab  Osteocalcin/metab  Mechanical stress  RNA  messenger/metab
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