| DNA去甲基化引起人二倍体成纤维细胞端区缩短并加速衰老进程 |
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| 引用本文： | 陈培利,童坦君,张宗玉.DNA去甲基化引起人二倍体成纤维细胞端区缩短并加速衰老进程[J].北京大学学报(医学版),2001,33(1):42-45. |
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| 作者姓名： | 陈培利 童坦君 张宗玉 |
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| 作者单位： | ^A北京大学基础医学院生物化学与分子生物学系,^B北京大学^C1447 |
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| 基金项目： | 国家自然科学基金;39930170; |
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| 摘 要： | 目的:研究DNA甲基化水平变化对细胞衰老进程和端区长度的影响.方法:从细胞形态和Southern印迹杂交测定端区长度两方面观察5-氮胞苷处理对人胚肺二倍体成纤维细胞衰老进程的影响.结果:5-氮胞苷处理使老年细胞衰老表型更为突出,其端区长度缩短较年轻细胞亦为显著(约为对照细胞的96%).结论:甲基化水平降低及其导致的端区缩短的共同作用可能在细胞衰老过程中发挥着重要的作用.
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| 关 键 词： | DNA 甲基化 端粒/药物作用 细胞衰老/药物作用 成纤维细胞/药物作用 |
| 文章编号： | 1000-1530(2001)01-0042-04 |
DNA demethylation leads to telomere shortening with accelerated aging process in human diploid fibroblasts |
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| CHEN Pei Li,TONG Tan Jun,ZHANG Zong Yu.DNA demethylation leads to telomere shortening with accelerated aging process in human diploid fibroblasts[J].Journal of Peking University:Health Sciences,2001,33(1):42-45. |
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| Authors: | CHEN Pei Li TONG Tan Jun ZHANG Zong Yu |
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| Abstract: | Objective: The effect of DNA demethylation on aging process and telomere length was studied. Methods: The effect of 5 aza cytidine treatment on aging process of human fetal lung diploid fibroblasts (2BS) was determined by cellular morphology and telomere length assayed by Southern blotting. Results: The results showed that DNA demethylation could advance the senescent phenotype more significantly in old cells than it did in young cells. Accordingly, telomere shortening seemed to be more apparent in old cells (about 96% of that of control). Conclusion: The decreased level of DNA methylation and the resulted telomere shortening may be important cooperative factors contributing to cellular aging process. |
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| Keywords: | DNA Methylation Telomere/drug eff Cellular aging/drug eff Fibroblasts/drug eff |
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