| 一氧化氮抑制单核-内皮细胞粘附的机制 |
| |
| 引用本文： | 霍玉庆,徐成斌,李帮清,王申五.一氧化氮抑制单核-内皮细胞粘附的机制[J].北京大学学报(医学版),1998,30(5):439-442. |
| |
| 作者姓名： | 霍玉庆 徐成斌 李帮清 王申五 |
| |
| 作者单位： | 1. 北京医科大学人民医院心血管内科,北京,100044
2. 北京医科大学人民医院中心实验室,北京,100044 |
| |
| 摘 要： | 目的：研究一氧化氮（ＮＯ）抑制单核内皮细胞粘附的机制。方法：以硝普钠作为ＮＯ的供体，用细胞粘附实验、流式细胞仪荧光强度测定、细胞ＥＬＩＳＡ、Ｎｏｒｔｈｅｒｎｂｌｏｔ等方法分别从细胞、蛋白表达、基因表达方面研究ＮＯ对内皮细胞粘附分子（ｖａｓｃｕｌａｒｃｅｌａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ，ＶＣＡＭ１）表达的影响。结果：ＮＯ抑制ＩＬ１α诱导的单核内皮细胞粘附，抑制ＩＬ１α诱发的ＶＣＡＭ１的表达并呈时间依赖性及浓度依赖性，ＮＯ在粘附分子ＶＣＡＭ１的基因表达水平上亦呈现抑制作用。结论：ＮＯ抑制ＶＣＡＭ１的表达是其抑制单核内皮细胞粘附及抗动脉粥样硬化的机制之一。
|
| 关 键 词： | 一氧化氮／药理学 内皮／药物作用 细胞粘附／药物作用 单核细胞／药物作用 |
INHIBITORY MECHANISM OF NITRIC OXIDE ON ADHESION BETWEEN MONOCYTE AND ENDOTHELIAL CELLS |
| |
| HUO Yuqing #,XU Chengbin,ZHANG Tong,LI Bangqing,WANG Shenwu.INHIBITORY MECHANISM OF NITRIC OXIDE ON ADHESION BETWEEN MONOCYTE AND ENDOTHELIAL CELLS[J].Journal of Peking University:Health Sciences,1998,30(5):439-442. |
| |
| Authors: | HUO Yuqing # XU Chengbin ZHANG Tong LI Bangqing WANG Shenwu |
| |
| Abstract: | Objective: To study the inhibitory mechanism of nitric oxide on adhesion between monocyte and endothelial cells. Methods:Cell adhesion,FACS, cell ELISA and Northern blot were applied to study effects of nitroprusside, a donor of nitric oxide, on expression of protein and mRNA of (vascular cell adhesion molecule, VCAM 1). Results: Expression of VCAM 1 protein and mRNA was inhibited by nitroprusside, in a dose time dependent manner. Conclusion: Inhibitory effect of nitric oxide on VCAM 1 is one of the mechanisms of its antiadhesion between monocyte and vascular endothelial cells,as well as that of its antiatherosclerosis. |
| |
| Keywords: | Nitric oxide/pharmacol Endothelium/drug eff Cell adhesion/drug eff Monocytes/drug eff |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
