不同牙周状况下唾液和龈沟液中弹性蛋白酶的含量

目的:比较不同牙周状况者唾液和龈沟液(gingival crevicular fluild,GCF)中的中性多形核粒细胞弹性蛋白酶(granulocyte elastase,EA)含量,并分析唾液与龈沟液EA水平的相关性。方法:采用底物法对牙周健康者(17例)、牙龈炎患者(14例)、慢性牙周炎(chronic periodontitis,CP)患者(24例)和侵袭性牙周炎(aggressive pe-riodontitis,AgP)患者(24例)的唾液和/或龈沟液样本中的EA含量进行测定分析。结果:AgP患者龈沟液EA水平高于CP患者(0.485 3±0.225 0 vs.0.288 4±0.193 1,P<0.01);牙周炎患者(AgP和CP)唾液EA水平高于牙周健康者和牙龈炎患者(0.844 5±0.660 6,0.637 3±0.648 9 vs.0.012 2±0.005 8,0.031 6±0.020 6;P均<0.001)。牙周炎患者唾液与龈沟液EA水平呈正相关关系(r=0.660)。结论:龈沟液EA的测定对不同类型牙周炎的诊断可起一定的辅助作用,唾液EA水平的测定可用于在人群普查中筛选牙周炎患者及进行全口牙周评估。

Granulocyte elastase levels in saliva and gingival crevicular fluid of subjects with various periodontal conditions

Objective: To compare the granulocyte elastase(EA) levels in saliva and/or gingival cre-vicular fluild(GCF) of subjects with various periodontal conditions and analyze the relation between EA levels in GCF and in saliva.Methods: GCF and salivary samples were collected from 17 subjects with healthy periodontium,14 with gingivitis,24 with chronic periodontitis(CP) and 24 with aggressive periodontitis(AgP).The EA levels in GCF and saliva were analyzed.Results: The GCF-EA level in AgP were significantly higher than that in CP(0.485 3±0.225 0 vs.0.288 4±0.193 1,P<0.01);the levels of EA in saliva of periodontitis patients(AgP and CP) were higher than those of healthy and gingivitis subjects(0.844 5±0.660 6,0.637 3±0.648 9 vs.0.031 6±0.020 6,0.012 2±0.005 8,P<0.001).A positive correlation was found between EA levels in saliva and those in GCF(r=0.660).Conclusion: GCF-EA level may serve as a marker for clinical assessment of periodontal conditions.The measurement of EA levels in saliva may facilitate to overall screen periodontitis patients in epidemiological study or to monitor periodontal conditions in clinical practice.