重组腺病毒介导血管内皮生长因子基因在骨髓间充质干细胞中的表达及其影响

目的观察重组腺病毒介导血管内皮生长因子基因(AdVEGF165)转染后大鼠骨髓间充质干细胞(BMSCs)中血管内皮生长因子的表达情况以及腺病毒载体对大鼠骨髓间充质干细胞的生长影响情况。方法体外培养大鼠骨髓间充质干细胞,用腺病毒载体转染BMSCs。采用ELISA法检测血管内皮生长子因子(VEGF)的表达和分泌。转染后用胰酶消化传代培养,MTT法绘制细胞生长曲线。结果AdVEGF165转染组上清液中VEGF蛋白表达量极显著高于空白对照组(P<0.01)。且AdVEGF165转染BMSCs后,生长曲线与正常培养细胞无显著差异(P>0.05)。结论重组腺病毒载体能够在BMSCs中介导VEGF的表达,且对BMSCs生长无影响。

Expression and impact of vascular endothelial growth factor gene which was mediated by recombination adenovirus in mesenchymal stem cells

Objective Recombinant Adenovirus mediate Vascular endothelial growth factor 165 gene (AdVEGF_165 ) transfer into bone mesenchymal stem cells (BMSCs), then to observe the change of growth of BMSCs after transfection and expression of VEGF. Methods Wistar rat BMSCs were cultured in vitro, AdVEGF_165 transfer it. ELISA method was applied to assay the expression and secretion of VEGF. BMSCs were digested and passage by trypsin, MTT method was applied to draw growth curve. Results Expression quantum of VEGF in AdVEGF_165 transfer group is higher than control group ( P < 0.01 ). Moreover, the BMSCs growth curve of transfer group and non-transfer group has not significant difference ( P > 0.05 ). Conclusion Recombination adenovirus vector can express VEGF in BMSCs, and doesn't affect BMSCs growth.