尼莫地平对脑缺血再灌注后大鼠血脑屏障通透性的影响

目的 研究尼莫地平对脑缺血再灌注后大鼠离屏障（ＢＢＢ）通透性的影响。方法 采用线栓法制作大鼠大脑中动脉闭塞的局灶性脑缺血模型。缺血１ｈ后再灌注，分别于血前及再灌注后静脉注射尼莫地平。采用免疫组织化学ＳＡＢＣ法，观察再灌注１２ｈ时，内生免疫球收Ｇ（ＩｇＧ）在脑组织中的表达，比较缺血前注射尼莫地平组，未注射尼莫地平组及再灌注后注射尼莫地平组三组大鼠脑组织中ＩｇＧ的表达水平，结果 未注射尼莫地平组大鼠，

Effects of nimodipine on blood-brain barrier permeability following cerebral ischemia/reperfusion injury in rats

Objective To investigate the effects of intravenous application of nimodipine on blood brain barrier(BBB)permeability following cerebral ischemia/reperfusion in rats.Methods Thread embolism method was used to develop the focal cerebral ischemic model of left middle cerebral artery occlusion for 1 h in rats.Reperfusion was performed 1 h after ischemia.Nimodipine was intravenously injected before ischemia and after reperfusion.By using SABC method,the expression of IgG in brain tissue was detected on the 12th h of reperfusion.The expression levels of IgG in rat brain tissue were compared among the group of nimodipine injection before ischemia(groupA),nom nimodipine injection group(groupB)and the group of nimodipine injection after reperfusion (group C).Results The expression of IgG was extensive in ischemic hemisphere on the 12th h of reperfusion in group B.The IgG expression was more extensive in group C than in group B.In group A,there was less expression of IgG.Conclusion Intravenous application of nimodipine following ischemia/reperfuison could accelerate BBB damage and increase its permeability.Intravenous application of nimodipine before cerebral ischemia could alleviate the damage of reperfusion to BBB.