RGD多肽修饰的改性PLGA仿生支架材料对骨髓间充质干细胞粘附性影响的研究

目的了解精氨酸甘氨酸天冬氨酸(RGD多肽)修饰是否能提高改性的PLGA仿生支架材料对骨髓间充质干细胞的粘附性。方法用异型双功能交联剂SulfoLCSPDP将GRGDSPC多肽共价结合到改性PLGA支架材料上,以未接多肽的改性PLGA材料做对照,取第三代MSC接种到材料上,培养4、12小时后沉淀法定量检测粘附的细胞数,培养24小时后摄光镜图像比较粘附细胞的数量和形态。结果培养4、12小时后实验组细胞粘附率分别为0.17±0.01、0.38±0.02,均极显著高于对照组的0.08±0.01、0.14±0.01(P<0.01),且24小时后细胞的粘附质量也较对照组为好。结论RGD多肽修饰的确能提高改性PLGA支架材料对骨髓间充质干细胞的粘附性。

MSC adhesion to PLGA modified with RGD peptide

Objective To study the effect of prepared PLGA modified with RGD peptide on the adhesion of MSC to this biomaterials.Methods Heterobifunctional cross-linker, Sulfo-LC-SPDP was used immobilize GRGDSPC on the surface of PLGA. The third generation MSCs were seeded onto the biomaterials and incubated for 4 h and 12 h at standard condition. At each point the number of adhesive cells was counted to evaluate the efficiency of cell adhesion, and the photo of adhesive cells cultured for 24 h was taken to evaluate the quality of cell adhesion.Results It was found at 4 h and 12 h the efficiency of cell adhesion in the modified PLGA was higher than in unmodified PLGA, and at 24 h the quality of cell adhesion in modified PLGA was better.Conclusion MSCs adhesion to PLGA can be enhanced by modifying the PLGA with RGD peptide.