| 熊果酸对乳腺癌SK-BR-3细胞增殖和凋亡的影响 |
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| 引用本文： | 徐新伟,郭玲玲,顾振纶,蒋小岗,周文轩,郭次仪.熊果酸对乳腺癌SK-BR-3细胞增殖和凋亡的影响[J].苏州大学学报(自然科学版),2009,29(1):68-70. |
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| 作者姓名： | 徐新伟 郭玲玲 顾振纶 蒋小岗 周文轩 郭次仪 |
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| 作者单位： | 徐新伟,XU Xin-wei(苏州大学附属第二医院病理科,江苏苏州215004;苏州中药研究所,江苏苏州215007);郭玲玲,GUO Ling-ling(苏州大学附属第二医院病理科,江苏苏州,215004);顾振纶,蒋小岗,周文轩,郭次仪,GU Zhen-lun,JIANG Xiao-gang,ZHOU Wen-xuan,GUO Ci-yi(苏州中药研究所,江苏苏州,215007)
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| 摘 要： | 目的探讨熊果酸（UA）对ER（-）、Her-2过表达的人乳腺癌SK-BR-3细胞增殖和凋亡的影响。方法利用噻唑蓝（MTT）比色法观察UA对SK-BR-3细胞增殖的影响。用Hoechst 33258荧光染色及电子显微镜观察凋亡细胞核形态变化。流式细胞仪检测UA对细胞周期的影响;DNA琼脂糖凝胶电泳观察DNA片段的断裂。结果UA明显抑制SK-BR-3细胞的增殖,差异有统计学意义（P〈0.05）,并呈时间和浓度依赖性。经UA处理后的实验组细胞呈现典型的凋亡核固缩表现,胞核呈致密浓染的颗粒状或块状荧光。流式细胞仪检测发现UA作用48h,后SK-BR-3细胞阻滞在G0/G1期,差异有统计学意义（均P〈0.05）。DNA琼脂糖凝胶电泳显示DNA大片段的断裂。结论UA在体外对SK-BR-3细胞具有抗增殖和诱导凋亡的作用。
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| 关 键 词： | 熊果酸 凋亡 细胞增殖 Her-2 |
Effects of Ursolic Acid on Proliferation and Apoptosis of SK-BR-3 Cells |
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| XU Xin-wei,GUO Ling-ling,GU Zhen-lun,JIANG Xiao-gang,ZHOU Wen-xuan,GUO Ci-yi.Effects of Ursolic Acid on Proliferation and Apoptosis of SK-BR-3 Cells[J].Suzhou University Journal of Medical Science,2009,29(1):68-70. |
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| Authors: | XU Xin-wei GUO Ling-ling GU Zhen-lun JIANG Xiao-gang ZHOU Wen-xuan GUO Ci-yi |
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| Institution: | XU Xin-wei, GUO Ling-lin, GU Zhen-lun, JIANG Xiao-gang ZHOU Wen-xuan,GUO Ci-yi(1.Dept of Pathology, the Second Hospital Affiliated to Soochow University, Jiangsu Suzhou 215004, China; 2.Suzhou Institute of Chinese Materia Medica, Jiangsu Suzhou 215007, China) |
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| Abstract: | Objective To investigate the inhibitory and apoptotic the proliferation of human breast cancer SK-BR-3 cells. Methods effect of ursolic acid （UA） on The effect of UA on the proliferation of SK-BR-3 cells was measured by MTT method. Hoechst 33258 fluorescent dying and electron microscope were used to observe UA induced morphological changes. The cell cycle was observed with flow cytometry. DNA fragmentation was observed by DNA agarose gel electrophoresis. Results UA inhibitied the growth of SK-BR-3 cells in a dose-and time-dependent manner. The morphological changes of apoptosis , such as nuclear fragmentation or condensed particles were identified after incubation with UA. Cell cycle analysis revealed that SK-BR-3 cells treated with UA for 48 h were blocked predominantly in G0/G1 phase （P〈0.05）. Large fragments of DNA were observed by DNA agarose gel electrophoresis. Conclusion UA has the effects of inhibition of SK-BR-3 cell proliferation |
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| Keywords: | Her-2 |
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