伤寒杆菌耐药质粒pRST98与细菌外膜泡的关系

目的：研究伤寒杆菌耐药质粒pRST98与细胞外膜泡（OMV）形成的关系。方法：将（1）携带pRST98的野生型耐药性伤寒杆菌－－ST1及ST2；（2）不含pRST98的野生型敏感伤寒杆菌－－ST3及ST4；（3）不含质粒的大肠杆菌－－E.coliK12W1485;(4)用十二烷基硫酸钠消除pRST8的突变体菌株－－ST1（R^-)及ST2（R^-)；和（5）经接合转移将pRST98导入敏感型伤寒杆菌及大肠杆菌的接合子－－pRST98/ST3、pRST98/ST4及pRST98的耐药伤寒杆菌消除pRST98前后，不含pRST98的的敏感伤寒杆菌和大鼠肝菌在导入该质粒前后，细菌形成OMV的数量和形态均无明显变化。结论：伤寒杆菌耐药质粒pRST98在体外与细菌外膜泡的形成未发现有明显关系。

Study on the Relationship between Drug Resistant Salmonella Typhi R Plasmid (pRST98) and Outer Membrane Vesicles of Bacteria

Objective To explore the relationship between drug resistant Salmonella.typhi R plasmid (pR ST98) and outer membrane vesicles (OMV) of bacteria.Methods Following strains were used in the experiment:(1) plasmid-containing wide-type S.typhi strain ST 1 and ST 2,(2) plasmid-free sensitive wide-type S.typhi strains ST 3,ST 4,(3) plasmid free sensitive E.coli K 12W 1485,(4) plasmid-cured strain ST 1(R -),ST 2(R -),(5) plasmid-introduced sensitive strains pR ST98/ST 3,pR ST98/ST 4,pR ST98/E.coliK 12W 1485.Their ability of OMV formation was compared by transmission electron microscope.Results The outer membrane vesicles of plasmid-free bacteria and those of bacteria harbouring pR ST98 were not found to be significantly different.Conclusion In vitro pR ST98 can not affect the ability of bacteria to form outer membrane vesicles.