全反式维甲酸对前列腺癌PC-3细胞Bcl-2、Bax表达的影响

目的 探讨全反式维甲酸（ATRA）对人雄激素非依赖性前列腺癌细胞株PC-3增殖的抑制作用及其Bel-2和Bax表达的影响。方法以不同浓度的ATRA处理PC-3细胞，甲基偶氮唑蓝（MTT）比色法检测细胞增殖抑制情况；流式细胞仪和免疫细胞化学法检测PC-3细胞Bax和Bcl-2的表达。结果ATRA浓度为（10^-6～10^-4）mol／L时，可明显抑制PC-3细胞的增殖（P〈0．01），并具有时间-剂量依赖性。ATRA处理后PC-3细胞Bcl-2表达下调，Bax表达上调。随ATRA浓度增加，Bcl-2／Bax的比值不同程度地下降（P〈0．05）。结论ATRA对PC-3细胞具有明显的增殖抑制作用，并且与ATRA下调Bcl-2表达、上调Bax表达有关。

Effects of All-trans-retinoic Acid on the Expression of Bcl-2 and Bax in Human Prostate Cancer PC-3 Cell Line

Objective To investigate the effects of all-transretinoic acid(ATRA) on the proliferation and the expression of Bcl-2 and Bax in human androgen-independent prostate cancer PC-3 cell line.Methods Human prostate cancer PC-3 cell line was cultured in medium containing in different of ATRA concentrations.Cell proliferating inhibition was tested by MTT assay.Flow cytometry and immunocytochemistry were used to analyze the expression of Bcl-2 and Bax in PC-3 cells.Results (ATRA) inhibited PC-3 cell proliferation in a dose-dependent and time-dependent manner(P<0.01).(ATRA) down-regulated the expression of Bcl-2 and up-regulated the expression of Bax in PC-3 cells.With the increase of ATRA concentration,the ratio of Bcl-2 to Bax decreased gradually.Conclusion ATRA can significantly inhibit the proliferation of PC-3 cells.And it is related with down-regulation of the expression of Bcl-2 and up-regulation of the expression of Bax.