雌激素受体2基因多态性与青年胃腺癌的关联分析

目的探讨雌激素受体2基因（ESR2）多态性与盐城地区青年胃腺癌的关联。方法应用聚合酶链反应-限制性片段长度多态性技术,对盐城地区100例青年胃腺癌患者（胃腺癌组）和100名青年胃溃疡患者（对照组）ESR2第5外显子RsaⅠ酶切多态和第8外显子AluⅠ酶切多态进行分析。结果（1）ESR2第8外显子AluⅠ酶切多态分析表明,胃腺癌组A等位基因频率明显高于对照组（P〈0.05,OR=1.975）,胃腺癌组含等位基因A的Aa＋AA基因型频率也明显高于对照组（P〈0.05,OR=2.144）;（2）两组ESR2第5外显子RsaⅠ酶切多态基因型rr、Rr、RR频率和等位基因r、R频率的比较,差异均无统计学意义（P〉0.05）。结论ESR2第8外显子AluⅠ多态可能与盐城地区青年胃腺癌易感性有关,含等位基因A的Aa和AA基因型可能增加青年胃腺癌发病风险;而ESR2第5外显子RsaⅠ多态与盐城地区青年胃腺癌发病风险无关联。

An Associated Analysis of Estrogen Receptor 2 Gene Polymorphism Linked with Gastric Adenocarcinoma

Objective To investigate the relationship of estrogen receptor 2 gene （ESR2） polymor-phism associated with gastric adenocarcinoma in Yancheng of China. Methods By polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method,the RsaⅠpolymorphism in exon 5 and the AluⅠpolymorphism in exon 8 of ESR2 were detected in 100 youths with gastric adenocarci-noma group and 100 youths with gastric ulcer（control group） in Yancheng. Results （1）The frequency of the allele A of AluⅠpolymorphism in exon 8 was significantly higher in gastric adenocarcinoma group than in the control group （P〈0.05,OR=1.975）,so was the frequency of the Aa＋AA geno-types （P〈0.05,OR=2.144）. （2）The genotype distributions（rr,Rr and RR） and allele frequencies （r and R） of RsaⅠpoly-morphism in exon 5 were not significantly different between the two groups （P〉0.05）. Conclusion The AluⅠpolymorphism in exon 8 of ESR2 may be associated with the susceptibility of gastric adenocarci-noma in Yancheng. The Aa＋AA geno-type significantly elevated the risk suffering from the gastric ade-nocarcinoma. The RsaⅠpolymorphism in exon 5 of ESR2 is not associated with the risk for puffering from the gastric adenocarcinoma in Yancheng.