| 利用ISSR分子法标记鉴别新会茶枝柑 |
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| 引用本文： | 邓锋,林向华,梁蔚阳.利用ISSR分子法标记鉴别新会茶枝柑[J].广东药学院学报,2012,28(4):396-399. |
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| 作者姓名： | 邓锋 林向华 梁蔚阳 |
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| 作者单位： | 1. 广东省食品药品检验所生检生化室,广东广州,510180
2. 中山大学孙逸仙纪念医院,广东广州,510120 |
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| 基金项目： | 广东省中医药管理局资助项目 |
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| 摘 要： | 目的研究利用ISSR分子标记方法在核酸分子水平上鉴别新会茶枝柑。方法从28条ISSR引物中筛选合适的引物,对从新会茶枝柑、砂糖桔、阳山桔、贡柑、芦柑共16个新鲜叶片样品中提取的总DNA进行PCR扩增及电泳分析,寻找特征位点。结果新会茶枝柑所有样品与其中2条ISSR引物扩增出较为明显的特征条带,可区别于其他品种。结论 ISSR作为一种简便有效的分子标记方法,可用于新会茶枝柑的鉴别。
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| 关 键 词： | 茶枝柑 ISSR 鉴别 |
Identification of Citrus Reticulata cv.Chachiensis by ISSR molecular markers |
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| DENG Feng
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LIN Xiang-hua
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LIANG Wei-yang.Identification of Citrus Reticulata cv.Chachiensis by ISSR molecular markers[J].Academic Journal of Guangdong College of Pharmacy,2012,28(4):396-399. |
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| Authors: | DENG Feng LIN Xiang-hua LIANG Wei-yang |
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| Institution: | 1(1.Biological Product & Biochemistry Department,Guangdong Provincial Institute for Drug Control,Guangzhou 510180,China;2.Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China) |
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| Abstract: | Objective To identify Citrus Reticulata cv.Chachiensis from Xinhui by ISSR molecular markers from different species of Citrus Reticulata.Methods 28 ISSR primers were analyzed by PCR with total DNA extracted from fresh leaves of 16 samples of Citrus Citrus Reticulata cv.Chachiensis,reticulata’Shiyueju’,reticulata’Yangshan’,reticulata’Gong’ and reticulata’Lu’.Results Two ISSR primers amplified from Citrus Reticulata cv.Chachiensis products showed different electrophoresis bands with other species of Citrus Reticulata.Conclusion ISSR method provides a quick,reliable molacular marker technique for identification of Citrus Reticulata cv.Chachiensis. |
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| Keywords: | Citrus Reticulata cv Chachiensis ISSR identification |
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