| 灵芝活性多糖的提取工艺研究 |
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| 引用本文： | 陈洁,雷林生,常花蕾.灵芝活性多糖的提取工艺研究[J].广东药学院学报,2009,25(4):348-351. |
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| 作者姓名： | 陈洁 雷林生 常花蕾 |
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| 作者单位： | 1. 广州市红十字会医院药剂科,广东广州,510220
2. 南方医科大学药学院药物研究所,广东广州,510515 |
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| 摘 要： | 目的确立水提醇沉法与超声提取法相结合提取灵芝活性多糖的最佳工艺。方法以脾细胞代谢MTT活力为评价指标，采用正交试验对超声时间、水提温度和水提时间等因素进行考察，寻求最佳的提取工艺。结果各因素对灵芝多糖脾细胞代谢MTT活力的影响大小排列为：超声时间〉水提温度〉水提时间，超声时间对灵芝多糖活性影响显著。提取最佳工艺为：超声提取时间20min，水提取温度80℃，水提取时间2h。结论本工艺为提取活性灵芝多糖提供了一定的参考。
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| 关 键 词： | 灵芝多糖 脾细胞 提取工艺 正交试验 |
Study on extraction of active polysaccharides from Ganoderma lucidum |
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| CHEN Jie,LEI Lin-sheng,CHANG Hua-lei.Study on extraction of active polysaccharides from Ganoderma lucidum[J].Academic Journal of Guangdong College of Pharmacy,2009,25(4):348-351. |
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| Authors: | CHEN Jie LEI Lin-sheng CHANG Hua-lei |
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| Institution: | CHEN Jie2,LEI Lin-sheng1,CHANG Hua-lei1(1.Institute of Pharmaceutical Sciences,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou,Guangdong 510515,China,2.Guangzhou Red Cross Hospital,Guangdong 510220,China) |
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| Abstract: | Objective To explore an optimum extraction procedure(water extraction-alcohol precipitation and ultrasonic extraction method) of active polysaccharides from Ganoderma lucidum.Methods By using colorimetric methyl thiazolyl tetrazolium (MTT) assay of splenic cells of mice as evaluation indicator,orthogonal test was conducted to evaluate ultrasonic extraction time,water extraction temperature,and water extraction time to explore the optimal extraction procedure.Results The main influencing factors of colorimet... |
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| Keywords: | Ganoderma lucidum polysaccharides splenic cells extraction procedure orthogonal experiments |
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