DM130型大孔树脂分离纯化野菊花总黄酮的工艺研究

目的 研究大孔吸附树脂从野菊花提取液中分离纯化总黄酮的工艺条件.方法 以总黄酮回收率为指标,采用静态和动态试验考察大孔吸附树脂对野菊仡总黄酮的分离纯化效果及影响因素.结果 DM130型大孔吸附树脂对野菊花总黄酮的最佳纯化工艺条件为:上柱样品溶液总黄酮质量浓度为2.78mg/mL,pH 3～4,以80%(体积分数)乙醇洗...

Purification of the total flavonoids from Chrysanthemum indicum with DM130 macroporous resin

Objective To investigate the conditions of purifying total flavonoids from Chrysanthem.indicma with macroporous adsorption resin. Methods Static and dynamic tests of macroporous resin wen enplosed to study on the purification effect and influencing factors with the recovery rate of total flavonoid Results The purification conditions of DM130 resin were as follows：the concentration of total 2.78 mg/mL at pH3 - 4,80% alcohol eluting for 5 times as much as the resin volume, and the velocity was 2 mL/min. The recovery of total flavonoids was 79. 19% , and the purification rate was 37.31%. Conclusion The optimum technology is stable and feasible, and DM130 resin is suitable for separating and purifying total flavonoids from Chrysanthemum indicum.