有机溶剂对乳化-溶剂挥发法制备PLGA纳米粒的影响

目的 以生物可降解材料聚（乳酸-羟基乙酸）共聚物(PLGA)为载体,采用乳化-溶剂挥发法制备包载荧光标记物香豆素-6的纳米粒,考察有机溶剂组成对纳米粒制备的影响.方法 选用不同配比的二氯甲烷与乙酸乙酯混合溶剂作为有机相制备香豆素-6-PLGA纳米粒,测定纳米粒粒径与包封率.结果 纳米粒粒径随着乙酸乙酯比例增加而减小,包...

Influence of organic solvent on preparation of PLGA nanoparticles by emulsification solvent evaporation method

Objective To prepare fluorescence marker coumarin-6 loaded nanoparticles by emulsification solvent evaporation method using the biodegradable material poly（lactic-co-glycolic acid）（PLGA） as carrier,and investigate the influence of organic solvent on nanoparticles′ formation.Methods Coumarin-6 loaded PLGA nanoparticles（coumarin-6-PLGA-NP） were prepared while organic phase was composed of dichloromethane（DCM） and ethyl acetate（EA） at different volume ratio.The entrapment efficiency and particle size of nanoparticles were determined by HPLC method and laser particle size analyzer.Results The nanoparticle size decreased with the increase of ethyl acetate,entrapment efficiency increased with the increase of dichloromethane.The particle size of nanoparticles and the entrapment efficiency was 128.7 nm and 35.9% respectively when combinations of DCM with EA were used at volume ratio of 70 ∶30.Conclusion Physical properties of organic solvents had significant effect on nanoparticles′ formation.PLGA nanoparticles prepared with combination of DCM and EA at ratio of 70 ∶30 exhibit narrow size distribution and higher entrapment efficiency.