HPLC法测定独子藤中雷公藤红素的含量

目的建立高效液相色谱法测定独子藤中雷公藤红素的含量,为雷公藤红素的药用植物资源开发提供依据。方法采用Supelco Discovery C18柱（25 cm×4.6 mm,5μm）,流动相为甲醇-5%（体积分数）H3PO4水溶液（体积比80∶20）,流速为1.0 mL.min-1,检测波长为426 nm。结果雷公藤红素在40～200μg.mL-1范围内与峰面积呈良好线性关系（r=0.999 1）。独子藤中雷公藤红素的平均质量分数为0.055 5%,雷公藤红素的平均回收率为97.6%,RSD=1.78%（n=6）。结论该方法简便、准确、灵敏度高、重现性好,可用于独子藤中雷公藤红素的含量测定;独子藤中雷公藤红素含量较高,可作为雷公藤红素的资源植物加以开发利用。

Determination of tripterine in Celastrus monospermus Roxb by HPLC

Objective To determine the content of tripterine in Celastrus monospermus Roxb by HPLC for developing the medicinal resources of celastrol.Methods An external standard method by HPLC with Discovery C18 column（25 cm×4.6 mm,5 μm） was employed.The mobile phase was consisted of methanol-5%（φ） phosphoric acid solution（80︰20）（ξ）at the flow rate of 1.0 mL·min-1.The detection wavelength was 426 nm.Results It showed good linearity in the range of 40-200 μg·mL-1（r=0.999 1）.The content of tripterine in Celastrus monospermus Roxb was 0.055 5%,and the average recovery rate of celastrol was 97.6%（RSD=1.78%,n=6）.Conclusion The method is simple and accurate,sensitive and reproducible and can be used in the quality control of Celastrus monospermus Roxb.The content of tripterine in Celastrus monospermus Roxb was high,which can be exploided as the herbal resource.