33种辛味中药挥发油皮肤细胞毒性与药性特征的关联性研究

目的 考察辛味中药挥发油的皮肤细胞毒性与来源中药药性特征的关联性。方法 测定33种中药挥发油的理化参数，采用人角质形成HaCaT细胞考察制得的33种中药挥发油的毒性，通过数据挖掘分析中药药性特征与挥发油皮肤细胞毒性之间的关联性。结果 采用线性典型相关分析法证明辛味中药归经与挥发油皮肤细胞毒性是高度相关的（P=0.0411<0.05），并且发现归肝、肺、胃、肾、大肠等经的挥发油皮肤细胞毒性小，归心、心包、脾、膀胱、三焦、胆等经的挥发油皮肤细胞毒性大。通过非线性典型相关分析法建立一种综合中药药性特征（四气、五味、归经）与挥发油皮肤细胞毒性相关的数学模型（P=0.0414<0.05），发现皮肤细胞毒性小的药性特征组合为凉+苦+归经（肝或肺或肾或大肠或胃），皮肤细胞毒性大的药性特征组合为热+甘+归经（心或心包或脾或膀胱或三焦或胆）。结论 药性特征尤其是归经影响辛味中药挥发油的皮肤细胞毒性。 

Study on Association Between Skin Cell Toxicity of 33 Pungent Essential Oils and Drug Property Characteristics of Chinese Materia Medica

OBJECTIVE To analyze the preliminary regulation between the skin cell toxicity of EOs and drug property characteristics of Chinese materia medica(CMM) on the same platform. METHODS The physicochemical parameters of 33 kinds of EOs were measured. MTT assays were applied to evaluate the cell toxicity of EOs on human HaCaT keratinocytes. Finally， data mining technique was applied to analyse experimental data for the purpose to illustrate the relationship between skin cell toxicity of EOs and drug properties of CMMs. RESULTS By non-linear canonical correlation analysis， high correlation（P=0.0411<0.05） was found between skin cell toxicity of EOs and channel tropism property of CMMs. EOs with the properties of liver， lung， stomach， kidney and large intestine channel possess lower skin cell toxicity. However， EOs with the properties of heart， pericardium， spleen， bladder， san-jiao and gall bladder channel possess higher skin cell toxicity. A mathematical model between drug property including four mature， five flavor， channel tropism of CMMs and skin cell toxicity of EOs was established based on non-linear canonical correlation analysis. In the model， significant correlation was found（P=0.0414<0.05）. EOs from CMMs with the properties of cool nature， bitter flavor plus liver or lung or kidney or large intestine channel might be superior in safety towards the skin cells. On contrary， EOs from CMMs with the properties of hot nature， sweet flavor plus heart or pericardium or spleen or bladder or san-jiao or gall bladder channel might be superior in toxicity towards the skin cells. CONCLUSION The skin cell toxicity of EOs is affected by drug property characteristics of CMMs， especially channel tropism property.