急性缺血性脑卒中患者血清淀粉样蛋白A及胱抑素C水平与病情程度相关性研究

目的观察急性缺血性脑卒中患者血清淀粉样蛋白A(SAA)及胱抑素C(CysC)水平变化,探讨急性缺血性脑卒中的发病机制及与SAA、CysC的关系。方法收集急性缺血性脑卒中患者66例作为观察组;健康体检者20例为健康对照组。2组均采集空腹肘静脉血,采用ELISA法测定血清SAA、CysC水平。观察组于入院24 h内进行美国国立卫生院卒中量表(NIHSS)评分,并对SAA、CysC水平与NIHSS评分的相关性进行分析。结果观察组血清SAA水平(2.293±1.067)mg/L vs.(0.405±0.189)mg/L]、CysC水平(1.924±0.693)mg/L vs.(0.681±0.173)mg/L]明显高于对照组(t=13.679、t=13.271,P均<0.05)。观察组按NIHSS评分分为轻型亚组17例(<4分),中型亚组45例(4¹5分),重型亚组4例(>15分);3个亚组间比较,SAA、CysC水平差异均有统计学意义(P<0.05),分别为重型亚组>中型亚组>轻型亚组。观察组血清SAA、CysC水平与NIHSS评分之间均呈正相关(r=0.981、r=0.934,P均<0.05)。结论急性缺血性脑卒中患者血清SAA、CysC水平明显高于正常健康人,血清SAA、CysC水平与NIHSS评分均呈正相关性。

The relevance of serum levels of amyloid A protein and changes of cystatin C in acute ischemic cerebrovascular disease

Objective To observe acute ischemic stroke patients serum amyloid A （SAA） and cystatin C （CysC） levels, to explore the relationship between the pathogenesis of acute ischemic stroke and SAA, CysC. Methods Collected patients with acute ischemic stroke in 66 cases as the observation group ; 20 cases of healthy people as healthy control group. The 2 groups were collected venous blood, using the method of ELISA for determination of serum SAA, CysC levels. The observation group in the hospital within 24 h were underwent National Institute of Health Stroke Scale（NIHSS） score evaluation, and the correlation between SAA, CysC level and NIHSS score were analyzed. Results Observation group＇S serum level of SAA （2.293 ±1.067） mg/L vs. （0.405 ±0. 189） mg/L] ,CysC （1.924 ±0.693） mg/L vs. （0.681 ±0. 173） mg/L] was significantly higher than the control group （ t = 13. 679, t = 13. 271, P 〈 0.05 ）. The observation group aceording to NiHSS score were divided into light sub-group with 17 eases （ 〈 4 points） , and medium subgroup of 45 eases （4- 15 points）, se- vere subgroup with 4 eases （ 〉 15 points） ; 3 subgroups＇SAA, CysC levels showed significant differences （ P 〈0.05）, re- spectively, as severe subgroup 〉 medium subgroup 〉 light subgroup. Observation group~ serum SAA, CysC levels were positively correlated with NIHSS score （ r = 0.981, r = 0. 934, P 〈 0.05 ）. Conclusion Acute ischemie cerebral stroke patients whose serum SAA, CysC levels were significantly higher than those of healthy people, the serum SAA and CysC level were positively eorrelated with NIHSS seore.