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参松养心胶囊对心室肌细胞钾通道的影响
引用本文:李宁,吴相锋,马克娟,赵新然,吴以岭,浦介麟.参松养心胶囊对心室肌细胞钾通道的影响[J].疑难病杂志,2007,6(3):133-137.
作者姓名:李宁  吴相锋  马克娟  赵新然  吴以岭  浦介麟
作者单位:1. 100037,北京,中国医学科学院,阜外心血管病医院,心律失常诊治中心
2. 病理与生理实验中心
3. 050035,石家庄,河北医科大学医药研究院
摘    要:目的观察参松养心胶囊干粉提取溶液对大鼠单个心室肌细胞内向整流钾电流(IK1)、瞬时外向钾电流(Ito)以及豚鼠心肌细胞延迟整流钾电流(Ik)的影响。方法用酶解法分离单个心室肌细胞,并用全细胞膜片钳记录技术。结果当药物浓度0.5%时,可以使大鼠心肌细胞IK1内向成分降低,使-100mV时IK1电流密度从(-10.8±1.8)pA/pF降至(-7.2±2.1)pA/pF,平均抑制率为(33.1±16.9)%(n=11,P<0.05),但不改变电流的翻转电位及整流特性。药物同时对Ito电流的瞬时成分有抑制作用,60mV时Ito电流密度从(-19.8±7.1)pA/pF降至(-10.0±3.9)pA/pF,平均抑制率为(50.6±10.8)%(n=6,P<0.05),稳态失活曲线左移,失活后恢复时间常数增大。药物浓度0.5%时,对Ik有电压依赖性抑制作用,50mV时对尾电流峰值的抑制率为(30.8±1.1)%(n=5,P<0.05)。结论参松养心胶囊干粉提取溶液对IK1,Ito和Ik均具有不同程度的阻滞作用。这种多离子通道阻滞作用不仅使参松养心胶囊具有广谱的抗心律失常疗效,减少致心律失常的副作用,对Ito的阻滞效应还将显示其独特的抑制2相折返的作用,值得对其作更深入和广泛的研究。

关 键 词:参松养心胶囊  钾离子通道  心律失常  心室肌细胞  
文章编号:1671-6450(2007)03-0133-05
收稿时间:2007-02-09
修稿时间:2007年2月9日

The effect of Shensongyangxin capsule on potassium channel currents in isolated ventricular myocytes
LI Ning,WU Xiang-feng,MA Ke-juan,et al..The effect of Shensongyangxin capsule on potassium channel currents in isolated ventricular myocytes[J].Journal of Difficult and Complicated Cases,2007,6(3):133-137.
Authors:LI Ning  WU Xiang-feng  MA Ke-juan  
Institution:LI Ning*,WU Xiang-feng,MA Ke-juan,et al.*Center for Arrhythmia Diagnosis and Treatment,Fuwai Cardiovascular Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100037,China
Abstract:Objective To investigate the effect of Shensonyangxincapsule on inward rectifier potassium currents (IK1),transient outward potassium current (Ito) and delayed rectifier current (Ik) in ventricular myocytes.Methods Whole cell patch-clamp technique was used to observe potassium currents in enzymatically isolated ventricular myocytes of rat or guinea-pig.Results Shensongyangxin capsule decreased the inward current of IK1 by 33.1% when test potential was -100 mV,but which had little effect on the reversal potential and the rectified property.The drug also inhibited the transient component of Ito by 50.6 % at 60 mV while which shifted the inactive curve negatively and prolonged the recovery time after channel inactivation.The density of Ik tail current was decreased by 30.77±1.11% (n=5,P<0.05) at 50 mV after the drug was used.Conclusion Shensongyangxin capsule can block Ik1,Ito and Ik,which may contribute to its anti-arrhythmic effect.The inhibitive effect on multiple channels might possess the broad-spectrum of antiarrhythmic effects and reduce the possibility of proarrhythmia.For the inhibition of Ito,which suggests the possible unique effect on suppression of phase 2 reentry, deserves more intensive investigation.
Keywords:Shensongyangxin capsule  Potassium channel  Arryhthmia  Ventricular myocytes  Rat
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