血管性痴呆小鼠海马NMDA受体免疫组织化学变化特征 |
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引用本文: | 翟金萍,陈莉,吕佩源,梁翠萍,樊敬峰,王伟斌,尹昱.血管性痴呆小鼠海马NMDA受体免疫组织化学变化特征[J].疑难病杂志,2005,4(1):1-3,F003. |
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作者姓名: | 翟金萍 陈莉 吕佩源 梁翠萍 樊敬峰 王伟斌 尹昱 |
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作者单位: | 1. 050051,河北省人民医院病理科 2. 河北省血液中心 3. 050051,河北省人民医院神经内科 |
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基金项目: | 河北省自然科学基金资助项目(301415) |
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摘 要: | 目的 观测血管性痴呆小鼠海马神经元N 甲基 D 天门冬氨酸 (NMDA)受体免疫组织化学变化特征,以探讨其在血管性痴呆发病中的作用。方法 双侧颈总动脉线结,反复缺血—再灌注法制备模型,利用跳台试验和水迷宫试验观测其行为学改变,采用免疫组织化学技术观测小鼠海马神经元N 甲基 D 天门冬氨酸受体蛋白的表达变化。结果 模型组小鼠学习、记忆成绩较假手术组明显降低(P<0. 01),其海马N 甲基 D 天门冬氨酸受体蛋白表达也明显下降 (P<0. 01)。结论 血管性痴呆小鼠学习、记忆成绩下降与其海马N 甲基 D 天门冬氨酸受体蛋白低水平表达有关。
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关 键 词: | 痴呆 血管性 N甲基D天门冬氨酸 免疫组织化学 小鼠 |
文章编号: | 1671-6450(2005)01-0001-03 |
修稿时间: | 2004年10月28 |
The features of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia by immunohistochemistry |
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Abstract: | Objective The characteristics of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia (VD ) were observed for the purpose of demonstrating its function in VD pathogenesis .Methods The mice were subjected for ischemia-reperfusion repe atedly in bilateral common carotid arteries by knotting to establish the VD mode ls.The behavior abnormalities were investigated by step-down test and water m aze test,and the N-methyl-D-aspartate receptor was observed by immunohistoch emistry technique.Results The grades of learning and memory in VD mice were worse than those in sham-operation group(P<0.01),and the exp ression of N-methyl-D-aspartate receptor in VD mice was also lower than that of sham-operation group(P<0.01).Conclusion The poor grade s of learning and memory in VD mice are related to the decrease of N-methyl-D -aspartate receptor in neuron of hippocampus. |
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Keywords: | Dementia vascular N-methyl-D-aspartate Immun ohistochemistry Mouse |
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