| Tumor Necrosis Factor-related Apoptosis Ligand Induces Apoptosis in Prostate Cancer PC-3M Cell Line |
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| 作者姓名： | 陈朝晖 王华芳 谷龙杰 叶哲伟 肖亚军 |
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| 作者单位： | [1]Department of Urology, Union Hospital. Tongji Medical College. Huazhong University of Science and Technology, Wuhan 430022, China [2]Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Scienceand Technology, Wuhan 430022, China |
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| 基金项目： | This project was supported by a grant from the NationalNatural Sciences Foundation of China （No. 30070756）. |
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| 摘 要： | Summary： To study the effect of tumor necrosis factor-related apoptosis inducing ligand （TRAIL） on PC-3M cell line, PC-3M cell line was incubated with gradient concentrations of TRAIL for 4-24 h. Annixin-V fluorescence staining and TUNEL method were employed to detect the apoptosis of PC-3M cells. The morphology of apoptotic PC-3M cells was observed by electron microscopy. The relationship between TRAIL concentrations and the percentage of apoptotic cells was evaluated by flow cytometry. The proliferation inhibitory ratio was calculated by using MTT colorimetry. Our results showed that apoptosis of PC-3M cells could be induced by treatment with TRAIL for at most 4 h. The results of flow cytometry and MTT colorimetry demonstrated a time-and concentration-dependent relationship between cell apoptosis rate and TRAIL concentration. It is concluded that apoptosis of PC-3M cells can be induced by TRAIL. Because of the selective killing effect of TRAIL on tumor cells, it may become a potential alternative for the treatment of advanced prostate cancer.
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| 关 键 词： | 肿瘤坏疽因子 配合基 前列腺癌 PC-3M细胞 |
| 收稿时间： | 2004-11-13 |
Tumor necrosis factor-related apoptosis ligand induces apoptosis in prostate cancer PC-3M cell line |
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| Chen Zhaohui,Wang Huafang,Gu Longjie,Ye Zhewei,Xiao Yajun.Tumor Necrosis Factor-related Apoptosis Ligand Induces Apoptosis in Prostate Cancer PC-3M Cell Line[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(4):442-444. |
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| Authors: | Chen Zhaohui Wang Huafang Gu Longjie Ye Zhewei Xiao Yajun |
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| Institution: | 1. Department of Urology, Union Hospital, Tongji Medical College, Huazhong University ofScience and Technology, Wuhan 430022, China
2. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary To study the effect of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) on PC-3M cell line, PC-3M cell line
was incubated with gradient concentrations of TRAIL for 4–24 h. Annixin-V fluorescence staining and TUNEL method were employed
to detect the apoptosis of PC-3M cells. The morphology of apoptotic PC-3M cells was observed by electron microscopy. The relationship
between TRAIL concentrations and the percentage of apoptotic cells was evaluated by flow cytometry. The proliferation inhibitory
ratio was calculated by using MTT colorimetry. Our results showed that apoptosis of PC-3M cells could be induced by treatment
with TRAIL for at most 4 h. The results of flow cytometry and MTT colorimetry demonstrated a time- and concentration-dependent
relationship between cell apoptosis rate and TRAIL concentration. It is concluded that apoptosis of PC-3M cells can be induced
by TRAIL. Because of the selective killing effect of TRAIL on tumor cells, it may become a potential alternative for the treatment
of advanced prostate cancer.
CHEN Zhaohui, male, born in 1974, M. D., Ph.D.
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30070756). |
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| Keywords: | TRAIL cell apoptosis prostate cancer PC-3M |
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