| Effect of Tumor Necrosis Factor-α on Acyl Coenzyme A： Cholesteryl Acyltransferase Activity and ACAT1 Gene Expression in THP-1 Macrophages |
| |
| 引用本文： | 何平,成蓓,王毅,王洪星.Effect of Tumor Necrosis Factor-α on Acyl Coenzyme A： Cholesteryl Acyltransferase Activity and ACAT1 Gene Expression in THP-1 Macrophages[J].华中科技大学学报(医学英德文版),2007,27(2):170-172. |
| |
| 作者姓名： | 何平 成蓓 王毅 王洪星 |
| |
| 作者单位： | Department of Gerontology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Gerontology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Cardiology the First People's Hospital Shanghai Jiao Tong University，Department of Molecular and Cell Biology Zeneca Pharmaceuticals，Wuhan 430022 China，Wuhan 430022 China，Shanghai 200080 China，Wilmington 19850 USA |
| |
| 基金项目： | This project was supported by a grant from the National Natural Sciences Foundation of China （No. 30170378）. |
| |
| 摘 要： | In order to explore the effect and mechanisms of tumor necrosis factor-α (TNF-α) on the activity of the acyl coenzyme A: cholesteryl acyltransferase (ACAT), THP-1 monocytes were cul- tured and induced to differentiate into macrophages with phorbol ester. TNF-α (60 ng/mL) was added at different time points into the macrophage-containing medium and the ACAT enzyme activity was measured by quantifying the incorporation of 1-14C oleoyl CoA into cholesteryl esters. The expres- sion of ACAT-1 protein and mRNA was respectively detected by Western blotting and RT-PCR in THP-1 macrophages 24 h after treatment with TNF-α (60 ng/mL). The results indicated that ACAT activity in THP-1 macrophages treated with TNF-α was increased in a time-dependent manner. The expression levels of ACAT-1 protein and mRNA were significantly increased in THP-1 macrophages after treatment with TNF-α (P<0.05). It was suggested that TNF-α could increase the activity of ACAT in THP-1 macrophages by up-regulating the expression of ACAT-1 gene.
|
| 关 键 词： | acyl coenzyme A: cholesteryl acyltransferase tumor necrosis factor-α macrophages cholesterol atherosclerosis |
| 收稿时间： | 2006-06-20 |
Effect of tumor necrosis factor-α on acyl coenzyme A: cholesteryl acyltransferase activity and ACAT1 gene expression in THP-1 macrophages |
| |
| He?Ping,Cheng?Bei,Wang?Yi,Wang?Hongxing.Effect of tumor necrosis factor-α on acyl coenzyme A: cholesteryl acyltransferase activity and ACAT1 gene expression in THP-1 macrophages[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(2):170-172. |
| |
| Authors: | He Ping Cheng Bei Wang Yi Wang Hongxing |
| |
| Institution: | (1) Department of Gerontology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China;(2) Department of Cardiology, the First People’s Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China;(3) Department of Molecular and Cell Biology, Zeneca Pharmaceuticals, Wilmington, 19850, USA |
| |
| Abstract: | Summary In order to explore the effect and mechanisms of tumor necrosis factor-α (TNF-α) on the activity of the acyl coenzyme A: cholesteryl acyltransferase (ACAT), THP-1 monocytes were cultured and induced to
differentiate into macrophages with phorbol ester. TNF-α (60 ng/mL) was added at different time points into the macrophage-containing medium and the ACAT enzyme activity was measured
by quantifying the incorporation of 1-14C] oleoyl CoA into cholesteryl esters. The expression of ACAT-1 protein and mRNA was respectively detected by Western blotting
and RT-PCR in THP-1 macrophages 24 h after treatment with TNF-α (60 ng/mL). The results indicated that ACAT activity in THP-1 macrophages treated with TNF-α was increased in a time-dependent manner. The expression levels of ACAT-1 protein and mRNA were significantly increased in
THP-1 macrophages after treatment with TNF-α (P<0.05). It was suggested that TNF-α could increase the activity of ACAT in THP-1 macrophages by up-regulating the expression of ACAT-1 gene.
HE Ping, female, born in 1974, Doctor in Charge
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30170378). |
| |
| Keywords: | acyl coenzyme A: cholesteryl acyltransferase tumor necrosis factor-α macrophages cholesterol atherosclerosis |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
