Dracorhodin perchlorate suppresses proliferation and induces apoptosis in human prostate cancer cell line PC-3 |
| |
Authors: | Yuanqiao He Wen Ju Hua Hao Qing Liu Lei Lv Fuqing Zeng |
| |
Institution: | 1Department of Laboratory Animal Science, Nanchang University, Nanchang 330006, China 2Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 3Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
Abstract: | The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined.
After administration of 10–80 μmol/L dracorhodin perchlorate for 12–48 h, cell viability of PC-3 cells was measured by MTT
colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine
orange-ethidium bromide fluorescent staining, Hoechst 33258 fluorescent staining, and flow cytometry (FCM) with annexin V-FITC/propidium
iodide dual staining. The results showed that dracorhodin perchlorate inhibited the growth of PC-3 in a dose- and time-dependent
manner. IC50 of dracorhodin perchlorate on PC-3 cells at 24 h was 40.18 μmol/L. Cell clone formation rate was decreased by
86% after treatment with 20 μmol/L of dracorhodin perchlorate. Some cells presented the characteristic apoptotic changes.
The cellular apoptotic rates induced by 10–40 μmol/L dracorhodin perchlorate for 24 h were 8.43% to 47.71% respectively. It
was concluded that dracorhodin perchlorate significantly inhibited the growth of PC-3 cells by suppressing proliferation and
inducing apoptosis of the cells. |
| |
Keywords: | dracorhodin perchlorate prostate cancer proliferation apoptosis |
本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录! |
|