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| Induction of Silencing Effect of Swedish Mutant Amyloid Precursor Protein by RNA interference | |
| 作者姓名： | 邱昕 陈国华 潘纪安 陈宇 张苏明 |
| 作者单位： | Department of Neurology Integrated Traditional Chinese and Western Medicine Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Neurology，Integrated Traditional Chinese and Western Medicine Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Modern Virology Research Center，College of Life Sciences，Wuhan University，Wuhan，430072，China，Modern Virology Research Center，College of Life Sciences，Wuhan University，Wuhan，430072，China，Department of Neurology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China |
| 基金项目： | We wish to thank Li Zhaoyang and Hussain Snar for helpful discussions and support. In addition, we gratefully acknowledge M. Lennart （The University of California, USA） for supplying the pXCJL-CMV constructs and Wang Jianzhi （Huazhong University of Science and Technology, Wuhan, China）. |
| 摘 要： | RNA interference (RNAi), as a novel technique of si- lencing genes, brings thrilling breakthrough in Alzheimer disease (AD) research. As a precursor of abnormally elevated Aβ, mutated APP is selectively inactivated by siRNA while wild type APP protein remains unaffected. Therefore, siRNA brings hope to gene therapy of familial AD (FAD) and further study of AD etiology. In order to observe the siRNA triggered silencing effect of APP, we constructed a green fluorescence protein (G… |
| 关 键 词： | 噪声抑制 瑞士 突变体 淀粉体 基因干预 |
| 收稿时间： | 2006-03-02 |
Induction of silencing effect of Swedish mutant amyloid precursor protein by RNA interference |
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| Xin Qiu,Guohua Chen,Ji’an Pan,Yu Chen,Suming Zhang.Induction of Silencing Effect of Swedish Mutant Amyloid Precursor Protein by RNA interference[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(4):399-401. | |
| Authors: | Xin Qiu Guohua Chen Ji’an Pan Yu Chen Suming Zhang |
| Institution: | 1. Department of Neurology, Integrated Traditional Chinese and Western Medicine Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2. Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, 430072, China 3. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | Over-expression of APP and Swedish mutation could cause some familial early onset AD. In this study, a primary screening was conducted of effective small interference RNAs (siRNAs) tar- geted wild type APP (APPwt) and Swedish mutant APP (APPswe). One siRNA targeting APPwt and the other siRNA targeting APPswe were designed. All these siRNAs were endogenously expressed by siRNAs expressing plasmids. COS-7 cells were transiently co-transfected with APP-GFP recombi- nant plasmids and siRNA expression vector. The silencing effect of each siRNA was quantitatively assessed by the level of expression of green fluorescent protein (GFP). It was found that the siRNAs silenced APPwt and APPswe to different degrees. siRNA directed against APPswe was more effective in suppressing the expression of fusion gene of APPswe than that of APPwt. The silencing effect of siRNA directed against APPswe indicating allele-specific silencing property of the siRNAs. There- fore, siRNAs directed against APP play an important role both in the therapeutic study of Alzheimer disease and functional exploration of APP gene. |
| Keywords: | amyloid precursor protein small interference RNA wild type APP Swedish mutant APP |
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