| The Effect of RhoC siRNA on the Invasiveness and Proliferation of Human Cervical Cancer Cell Line SiHa Cells |
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| 作者姓名： | 贺晓琪 钱颖 蔡惠兰 王泽华 |
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| 作者单位： | Department of Obstetrics and Gynecology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology |
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| 摘 要： | This study investigated the effect of RhoC GTPase on the proliferation and metastasis of cervical cancer cells, SiHa cells, in vitro. RhoC siRNA was introduced into SiHa cells to silence the RhoC gene. The mRNA and protein expression of RhoC, before and after RhoC siRNA transfection, was examined by RT-PCR and Western blotting, respectively. The proliferation and apoptosis of SiHa cells were examined by MTT assay and flow cytometry （FACS）, respectively. Adhesive rate was evaluated by Matrigel adhesive assay, and the invasive capability and migration capability were assessed by transwell invasive assay and migration assay, respectively. The results showed that after the RhoC siRNA transfection, the mRNA and protein expression of RhoC was down-regulated in SiHa cells. The down-regulation of RhoC GTPase did not affect the cell proliferation and apoptosis （P〉0.05）, but it did suppress SiHa cells＇ adhesion to matrigel （P〈0.01）, the invasive capability （P〈0.01） and the migration capability （P〈0.01）. It was concluded that RhoC obviously promotes the adhesion, invasion and migration of SiHa cells in vitro, but not proliferation and apoptosis, suggesting that RhoC plays an important role in the progression in cervical cancer.
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| 关 键 词： | RhoC基因 SiHa细胞 宫颈癌细胞系 siRNA 细胞增殖 侵袭能力 Western印迹 Transwell |
| 收稿时间： | 15 November 2006 |
The effect of RhoC siRNA on the invasiveness and proliferation of human cervical cancer cell line SiHa cells |
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| Xiaoqi He,Ying Qian,Huilan Cai,Zehua Wang.The Effect of RhoC siRNA on the Invasiveness and Proliferation of Human Cervical Cancer Cell Line SiHa Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(6):665-669. |
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| Authors: | Xiaoqi He Ying Qian Huilan Cai Zehua Wang |
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| Institution: | Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary This study investigated the effect of RhoC GTPase on the proliferation and metastasis of cervical cancer cells, SiHa cells,
in vitro. RhoC siRNA was introduced into SiHa cells to silence the RhoC gene. The mRNA and protein expression of RhoC, before and
after RhoC siRNA transfection, was examined by RT-PCR and Western blotting, respectively. The proliferation and apoptosis
of SiHa cells were examined by MTT assay and flow cytometry (FACS), respectively. Adhesive rate was evaluated by Matrigel
adhesive assay, and the invasive capability and migration capability were assessed by transwell invasive assay and migration
assay, respectively. The results showed that after the RhoC siRNA transfection, the mRNA and protein expression of RhoC was
down-regulated in SiHa cells. The down-regulation of RhoC GTPase did not affect the cell proliferation and apoptosis (P>0.05), but it did suppress SiHa cells’ adhesion to matrigel (P<0.01), the invasive capability (P<0.01) and the migration capability (P<0.01). It was concluded that RhoC obviously promotes the adhesion, invasion and migration of SiHa cells in vitro, but not proliferation and apoptosis, suggesting that RhoC plays an important role in the progression in cervical cancer.
Xiaoqi He, female, born in 1974, M.D. |
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| Keywords: | RhoC GTPase siRNA proliferation invasion |
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