| Smac/DIABLO Promotes Mitomycin C-induced Apoptosis of Bladder Cancer T24 Cells |
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| 作者姓名： | 汪良 曾甫清 郑丽端 童强松 |
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| 作者单位： | Department of Urology，Department of Urology，Department of pathology. Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China，Department of Urology |
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| 基金项目： | ThisprojectwassupportedbyagrantfromNationalNaturalSciencesFoundationofChina(No.30271301). |
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| 摘 要： | Thesecondmitochondria derivedactivatorofcaspase(Smac)ordirectIAPbindingproteinwithlowpl(DIABLO),amitochondrialproteinthatisreleasedtogetherwithcytochromeCfromthemi tochondriainresponsetoapoptoticstimuli,wasrecentlyfoundtopromotecaspaseactivationbybindingandneutralizingtheinhibitorofapoptosisproteins(IAPs)1-3].Inthisstudy,inordertoex plorethepossibilityofSmac/DIABLOasanoveltargetofgenetherapyforbladdercancer,theeffectoftheSmacexpressiononthemitomycinC(MMC)inducedapoptosisofthebladder…
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| 关 键 词： | 丝裂霉素 抗肿瘤抗生素 细胞凋亡 膀胱癌 |
| 收稿时间： | 2006-03-18 |
Smac/DIABLO promotes mitomycin C-induced apoptosis of bladder cancer T24 cells |
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| Wang Liang,Zeng Fuqing,Zheng Liduan,Tong Qiangsong.Smac/DIABLO Promotes Mitomycin C-induced Apoptosis of Bladder Cancer T24 Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(3):317-318. |
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| Authors: | Wang Liang Zeng Fuqing Zheng Liduan Tong Qiangsong |
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| Institution: | 1. Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | The enhancing effects of Smac gene on the mitomycin C-induced apoptosis of the bladder cancer cell line T24 were investigated. The Smac gene was transfected into bladder cancer cell line T24 under the induction of liposome. The intrinsic Smac level was detected by using immunohistochemistry and RT-PCR. The in vitro cellular growth activities were assayed by MTT colorimetry. Apoptosis was assayed by the flow cytometry. The results showed that as compared with the control cells, the apoptosis rate of T24 cells induced by mitomycin C was enhanced by transfected Smac gene. Flow cytometry revealed that, the apoptosis rate was 18.84 % and 33.52 %, and 10.72 % and 26.24 % respectively in blank and transfected cells treated with 0.05 or 0.005 mg/mL mitomycin C (P<0.05). It was concluded that Smac could enhance the apoptosis of T24 by mitomycin C, which could provide a useful experimental evidence for bladder cancer therapy. |
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| Keywords: | Smac gene bladder cancer mitomycin C apoptosis |
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