| Differentiation of Bone Marrow Mesenchymal Cells to Neural Cells |
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| 作者姓名： | 吴永超 郑启新 郭晓东 谢宗平 王运涛 郝杰 |
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| 作者单位： | Department of Orthopedics, Union HospitaL, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China |
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| 摘 要： | Summary： To explore the possibility and condition of differentiation of bone marrow mesenchymal ceils （BMSCs） to neural ceils in vitro, BMSCs from whole bone marrow of rats were cultured. The BMSCs of passage 3 were identified with immunocytochemical staining of CD44 （ ＋ ）, CD71 （ ＋ ） and CD45（-）, There were type I and type H ceils in BMSCs. Type I BMSCs were spindlehaped and strong positive in immunocytochemical staining of CD44 and CD71, whereas flat and big type H BMSCs were lightly stained. The BMSCs of same passage were induced to differentiate into neural ceils by β-mercaptoethanol （BME）. After induction by BME, the type I BMSCs withdrew to form neuron-like round soma and axon-like and dendrite-like processes, and were stained positively for neurofilament （NF）. The type H BMSCs did not change in the BME medium and were negatively or slightly stained of NF.
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| 关 键 词： | 骨髓间充质干细胞 神经细胞 神经轴突 脊髓损伤 |
| 收稿时间： | 2004-05-14 |
Differentiation of bone marrow mesenchymal cells to neural cells |
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| Wu Yongchao,Zheng Qixin,Guo Xiaodong,Xie Zhongping,Wang Yuntao,Hao Jie.Differentiation of Bone Marrow Mesenchymal Cells to Neural Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(5):488-490. |
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| Authors: | Wu Yongchao Zheng Qixin Guo Xiaodong Xie Zhongping Wang Yuntao Hao Jie |
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| Institution: | Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China |
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| Abstract: | Summary To explore the possibility and condition of differentiation of bone marrow mesenchymal cells (BMSCs) to neural cellsin vitro, BMSCs from whole bone marrow of rats were cultured. The BMSCs of passage 3 were identified with immunocytochemical staining
of CD44(+), CD71(+) and CD45(−). There were type I and type II cells in BMSCs. Type I BMSCs were spindle-shaped and strong
positive in immunocytochemical staining of CD44 and CD71, whereas flat and big type II BMSCs were lightly stained. The BMSCs
of same passage were induced to differentiate into neural cells by β-mercaptoethanol (BME). After induction by BME, the type
I BMSCs withdrew to form neuron-like round soma and axon-like and dendrite-like processes, and were stained positively for
neurofilament (NF). The type II BMSCs did not change in the BME medium and were negatively or slightly stained of NF.
WU Yongchao, male, born in 1971, M. D., Ph. D. |
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| Keywords: | bone mesenchymal stem cells cell differentiation neuron |
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